Key Laboratory for the Information System of Mountainous Areas and Protection of the Ecological Environment, Guizhou Normal University, Guiyang, Guizhou, P. R. China.
Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, P. R. China.
J Sep Sci. 2019 Nov;42(21):3281-3292. doi: 10.1002/jssc.201900531. Epub 2019 Sep 10.
To evaluate the xanthine oxidase inhibitory activity of the chemical constituents of Ligustrum lucidum in vitro, the spectrum-effect relationship was investigated. The high-performance liquid chromatography fingerprint was established by ultraviolet spectrophotometry, and the xanthine oxidase inhibitory activity was tested in vitro by a high-throughput screening method. Cluster analysis, principal component analysis, gray correlation analysis, and partial least squares regression were used to explore the spectrum-effect relationships. Sixty batches of Ligustrum lucidum were collected from 16 provinces for testing. The results revealed differences among the batches of medicinal materials, and the similarity score was between 0.635 and 0.968. Thirty-three characteristic peaks (1-33) were calibrated by fingerprint evaluation software for traditional Chinese medicine. The spectrum-effect relationship study further revealed that the contents of peaks 1, 2, 4, 5, 6, 7, 14, 17, 25, 28, 31, and 33, which are potentially critical ingredients for quality control of Ligustrum lucidum fruit, were highly correlated with the inhibition of xanthine oxidase activity.
为了评估女贞子化学成分的黄嘌呤氧化酶抑制活性，进行了谱效关系研究。采用紫外分光光度法建立高效液相色谱指纹图谱，采用高通量筛选法进行体外黄嘌呤氧化酶抑制活性试验。采用聚类分析、主成分分析、灰色关联分析和偏最小二乘回归法探讨谱效关系。从 16 个省收集了 60 批女贞子进行测试。结果表明，药材批次之间存在差异，相似度评分为 0.635 至 0.968。通过中药指纹图谱评价软件对 33 个特征峰（1-33）进行了标定。谱效关系研究进一步表明，女贞子果实质量控制的潜在关键成分，即峰 1、2、4、5、6、7、14、17、25、28、31 和 33 的含量与黄嘌呤氧化酶活性抑制高度相关。
