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抗寒酵母(异常威克汉姆酵母)菌株对冷藏鱼糜的抗氧化活性的代谢产物分析。

Metabolites analysis for cold-resistant yeast (Wickerhamomyces anomalus) strains own antioxidant activity on cold stored fish mince.

机构信息

College of Food Science and Engineering, Central South University of forestry and Technology, Changsha 410004, China.

College of Food Science and Engineering, Central South University of forestry and Technology, Changsha 410004, China.

出版信息

Food Chem. 2020 Jan 15;303:125368. doi: 10.1016/j.foodchem.2019.125368. Epub 2019 Aug 13.

Abstract

The effect of eight cold-resistant yeast strains (J3, J7, J8, J9, J12, J15, J18, and J25) of Wickerhamomyces anomalus on the lipid oxidation of cold stored fish mince (4 °C) were investigated. And the metabolites of these yeast were determined with gas chromatography-mass spectrometry. These strains could effectively inhibit the increase of hydroperoxides value (p < 0.05), and the inhibiting rate was positively correlated with the content of isolongifolene, xylitol, turanose, thymol-glucoside, and uridine. Especially, the J3, J7, J8, J9, J12, and J18 could eliminate a large part of thiobarbituric acid reactive substances (TBARS) (p < 0.05), the eliminating rate was proportionate to the aldehyde dehydrogenase activity. Several bacteriostatic metabolites were detected: thymol-glucoside, 2-phenylethanol, cedro, and 2,4-bis (1,1-dimethylethyl) phenol. In addition, W. anomalus produced many metabolites with fruit and floral notes. In conclusion, cold-resistant W. anomalus strains own antioxidant activity were potential new bio-preservatives in the cold storage of muscle products.

摘要

研究了 8 株威克汉姆酵母(J3、J7、J8、J9、J12、J15、J18 和 J25)对冷藏鱼糜(4°C)脂质氧化的影响。并采用气相色谱-质谱法测定了这些酵母的代谢产物。这些菌株能有效抑制过氧化物值的增加(p<0.05),抑制率与异长叶烯、木糖醇、松三糖、麝香草酚葡萄糖苷和尿苷的含量呈正相关。特别是 J3、J7、J8、J9、J12 和 J18 能消除大部分硫代巴比妥酸反应物(TBARS)(p<0.05),消除率与醛脱氢酶活性成正比。检测到几种抑菌代谢产物:麝香草酚葡萄糖苷、2-苯乙醇、雪松醇和 2,4-双(1,1-二甲基乙基)苯酚。此外,威克汉姆酵母还产生了许多具有水果和花香的代谢产物。综上所述,具有抗氧化活性的耐冷威克汉姆酵母菌株可能是肌肉制品冷藏的新型生物防腐剂。

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