Department of Environment and Safety Engineering, Taiyuan Institute of Technology, Taiyuan 030008, China.
SEM Bio-Engineering Technology Co., Ltd., Dalian 116600, China.
Molecules. 2021 Dec 24;27(1):113. doi: 10.3390/molecules27010113.
In the present work, the extraction process of total flavonoids (TFs) from flowers by ultrasound-assisted extraction was optimized under the response surface methodology (RSM) on the basis of single-factor experiments. The optimal extraction conditions were as follows: ethanol concentration of 80%, solid-liquid ratio of 1:37 (g/mL), temperature of 84 °C, and extraction time of 1 h. Under the optimized conditions, the extraction yield of the TFs was 3.956 ± 0.04%. The radical scavenging capacities of TFs against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) were much greater than that of rutin. The results of antibacterial experiments indicated that the TFs displayed strong inhibitory activities on , and . Therefore, flowers can be used as a novel source of natural flavonoids, and the TFs have potential applications as natural antioxidants or antibacterial agents in the food and pharmaceutical industries.
在本工作中，基于单因素实验，利用响应面法优化了超声辅助提取花中总黄酮（TFs）的提取工艺。最佳提取条件为：乙醇浓度 80%，固液比 1:37（g/mL），温度 84°C，提取时间 1 h。在优化条件下，TFs 的提取率为 3.956±0.04%。TFs 对 2,2-二苯基-1-苦基肼（DPPH）和 2,2-连氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）的自由基清除能力均强于芦丁。抑菌实验结果表明，TFs 对 、 和 表现出较强的抑制活性。因此，花可以作为天然类黄酮的新型来源，TFs 有望作为天然抗氧化剂或抗菌剂应用于食品和制药工业。
