利用CRISPR/Cas9从人胚胎干细胞中生成TLR7杂合敲除系（WAe009-A-18）。
Generation of a TLR7 heterozygous knockout line (WAe009-A-18) from human embryonic stem cells using CRISPR/Cas9.
作者信息
Seo Hyang-Hee, Han Hyo-Won, Kim Jung-Hyun
机构信息
Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea.
Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea.
出版信息
Stem Cell Res. 2020 May;45:101735. doi: 10.1016/j.scr.2020.101735. Epub 2020 Mar 13.
Toll-like receptor 7 (TLR7) is a member of the toll-like receptor (TLR) family that is essential in the innate immune system. In this study, we established a heterozygous TLR7 knockout H9 cell line using CRISPR/Cas9. TLR7 knockout H9 cells maintained their pluripotency and exhibited the ability to differentiate into the three germ layers without any karyotype abnormalities.
Toll样受体7（TLR7）是Toll样受体（TLR）家族的成员，在先天免疫系统中至关重要。在本研究中，我们使用CRISPR/Cas9建立了杂合TLR7基因敲除的H9细胞系。TLR7基因敲除的H9细胞保持了其多能性，并表现出分化为三个胚层的能力，且没有任何核型异常。
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