Green T L, Meyer J R, Daniels T E, Greenspan J S, de Souza Y, Kramer R H
Department of Stomatology, School of Dentistry, University of California, San Francisco 94143-0424.
J Oral Pathol. 1988 Jul;17(6):266-72. doi: 10.1111/j.1600-0714.1988.tb01535.x.
Early-stage lesions of Kaposi's sarcoma (KS) are composed of single-layered, highly flattened cells lining collagen bundles, whereas late-stage lesions contain densely packed, spindle-shaped cells. We examined the progression of KS lesions in oral mucosa and lymph nodes from patients with AIDS, using antibodies specific for blood vascular endothelial cells (Factor VIII-related antigen) and their basement membrane (Type IV collagen and laminin). In addition, the plant lectin Ulex europaeus, which selectively stains blood vessels, was also used. In early-stage KS lesions, fibronectin, laminin and Type IV collagen were co-distributed at the interface between KS cells and collagen bundles; Factor VIII-related antigen and Ulex europaeus lectin staining was present in vascular channels and in the KS cells. However, in late-stage lesions, few if any KS cells stained with antibody to Factor VIII-associated antigen, although endothelial cells lining blood vessels were positive. Strong staining for laminin and Type IV collagen was present in a pericellular pattern throughout the nodular late-stage lesions. Since lymphatic capillary endothelium does not produce basement-membrane-specific macromolecules, these results support the conclusion that KS cells are related to blood vascular endothelium but eventually lose certain endothelium-specific markers as the cells are transformed into the spindle-shaped cell type.
卡波西肉瘤（KS）的早期病变由排列在胶原束上的单层高度扁平细胞组成，而晚期病变则含有密集排列的梭形细胞。我们使用针对血管内皮细胞（VIII因子相关抗原）及其基底膜（IV型胶原和层粘连蛋白）的特异性抗体，研究了艾滋病患者口腔黏膜和淋巴结中KS病变的进展情况。此外，还使用了能选择性染色血管的植物凝集素欧洲荆豆。在KS早期病变中，纤连蛋白、层粘连蛋白和IV型胶原在KS细胞与胶原束的界面处共同分布；VIII因子相关抗原和欧洲荆豆凝集素染色出现在血管通道和KS细胞中。然而，在晚期病变中，尽管血管内皮细胞呈阳性，但很少有KS细胞被VIII因子相关抗原抗体染色。在整个结节状晚期病变中，层粘连蛋白和IV型胶原以细胞周围模式呈现强染色。由于淋巴管内皮细胞不产生基底膜特异性大分子，这些结果支持以下结论：KS细胞与血管内皮细胞相关，但随着细胞转变为梭形细胞类型，最终会失去某些内皮细胞特异性标志物。
