Kramer R H, Fuh G M, Hwang C B, Conant M A, Greenspan J S
J Invest Dermatol. 1985 Jun;84(6):516-20. doi: 10.1111/1523-1747.ep12273506.
Nearly one-third of all young homosexual men diagnosed as having acquired immune-deficiency syndrome (AIDS) develop a disseminated form of dermal Kaposi's sarcoma (KS). Although the histogenesis of KS cells is unclear, certain evidence suggests that the aberrant cells are of endothelial derivation. We have examined the presence and distribution of connective tissue-specific and basement membrane-specific macromolecules by indirect immunofluorescence and immunoperoxidase staining of frozen sections in early cutaneous lesions of KS from individuals with AIDS. The KS cells typically line the spaces between collagen bundles of the reticular dermis. When stained for the connective tissue-specific glycoprotein fibronectin, all Kaposi's sarcoma lesions showed an intense staining pattern, revealing a complex array of linear deposits of antigen that outlined the exterior surface of the collagen bundles. Antibodies to laminin and type IV collagen, both basement membrane-specific macromolecules, produced an intense staining pattern similar to that found with the anti-fibronectin antiserum, indicating that all 3 antigens are closely codistributed. In contrast, antibodies to type I collagen, the major collagen of the dermis, uniformly stained the collagen bundles in the KS lesions and in the normal control skin. Antiserum to factor VIII-associated antigen, an antigen specific to blood vascular endothelium, frequently stained the KS lesions but the staining pattern was diffuse and of variable intensity. The results suggest that KS cells are derived from the endothelium of the blood microvasculature and maintain their secretory phenotype of secreting basement membrane-specific macromolecules.
在所有被诊断患有获得性免疫缺陷综合征（艾滋病）的年轻同性恋男性中，近三分之一会发展为播散性皮肤卡波西肉瘤（KS）。尽管KS细胞的组织发生尚不清楚，但某些证据表明异常细胞源自内皮细胞。我们通过间接免疫荧光和免疫过氧化物酶染色，对艾滋病患者KS早期皮肤病变的冰冻切片进行了检查，以确定结缔组织特异性和基底膜特异性大分子的存在和分布。KS细胞通常排列在网状真皮胶原束之间的间隙中。当用结缔组织特异性糖蛋白纤连蛋白染色时，所有卡波西肉瘤病变均呈现强烈的染色模式，显示出一系列复杂的线性抗原沉积物，勾勒出胶原束的外表面。针对层粘连蛋白和IV型胶原的抗体，这两种都是基底膜特异性大分子，产生了与抗纤连蛋白抗血清相似的强烈染色模式，表明这三种抗原紧密共分布。相比之下，针对I型胶原（真皮的主要胶原）的抗体，均匀地染色了KS病变和正常对照皮肤中的胶原束。针对VIII因子相关抗原的抗血清，一种血管内皮特异性抗原，经常使KS病变染色，但染色模式是弥漫性的且强度可变。结果表明，KS细胞源自血液微血管的内皮细胞，并维持其分泌基底膜特异性大分子的分泌表型。
