Jones R R, Spaull J, Spry C, Jones E W
J Clin Pathol. 1986 Jul;39(7):742-9. doi: 10.1136/jcp.39.7.742.
Immunohistochemical studies were performed in thirty skin biopsies from patients with Kaposi's sarcoma, who did and did not have the acquired immune deficiency syndrome (AIDS). Tumour histogenesis was rigorously tested using a battery of endothelial cell markers, which included two new monoclonal antibodies, EN4 and PAL E. These are both specific for endothelial cells and can be visualised in appropriately fixed paraffin embedded tissue. Whereas EN4 labels all endothelial cells, PAL E is negative in endothelium of lymphatic derivation. Lectin binding with Ulex europaeus agglutinin 1 (UEA-1) and the presence of factor VIII related antigen (FVIIIRA) and laminin were also examined. In nodular lesions of Kaposi's sarcoma the spindle cell areas were positive with EN4 and UEA-1, negative with PAL E, and showed focal staining for FVIIIRA and laminin. These results confirm that the tumour is of endothelial cell origin. Six patch stage lesions showed a network of angulated spaces, lined by cells that were positive with EN4 and UEA-1, negative with PAL E and anti-FVIIIRA, and showed only weak staining for laminin. This pattern was observed in both AIDS and non-AIDS related cases and strongly favours a lymphatic derivation for the tumour. This has important implications as it suggests that lymphatic endothelium may have special characteristics that lead to neoplastic transformation in patients with retrovirus infection.
对30例卡波西肉瘤患者的皮肤活检组织进行了免疫组织化学研究，这些患者有的患有、有的未患获得性免疫缺陷综合征（艾滋病）。使用一系列内皮细胞标志物对肿瘤组织发生进行了严格检测，其中包括两种新的单克隆抗体EN4和PAL E。这两种抗体均对内皮细胞具有特异性，且能在适当固定的石蜡包埋组织中显现出来。EN4可标记所有内皮细胞，而PAL E在淋巴源性内皮细胞中呈阴性。还检测了荆豆凝集素1（UEA-1）的凝集素结合情况以及因子VIII相关抗原（FVIIIRA）和层粘连蛋白的存在情况。在卡波西肉瘤的结节性病变中，梭形细胞区域EN4和UEA-1呈阳性，PAL E呈阴性，并显示FVIIIRA和层粘连蛋白的局灶性染色。这些结果证实该肿瘤起源于内皮细胞。6例斑片期病变显示有一个由成角间隙构成的网络，间隙内衬的细胞EN4和UEA-1呈阳性，PAL E和抗FVIIIRA呈阴性，且层粘连蛋白仅呈弱阳性染色。这种模式在艾滋病相关和非艾滋病相关病例中均有观察到，强烈提示该肿瘤起源于淋巴管。这具有重要意义，因为它表明淋巴内皮细胞可能具有特殊特征，导致逆转录病毒感染患者发生肿瘤转化。
