Departments of Endoscopy Center, The First Hospital of Hebei Medical University, 050031, Shijiazhuang, Hebei, China.
Departments of General Surgery, The First Hospital of Hebei Medical University, 050031 Shijiazhuang, Hebei, China.
Exp Cell Res. 2019 Nov 1;384(1):111568. doi: 10.1016/j.yexcr.2019.111568. Epub 2019 Aug 22.
Chemotherapy and radiotherapy are main adjuvant therapies for the treatment of gastric cancer, the treatment effects are individual difference, but the specific mechanism is unknown. CyTOF 2 mass cytometer (CyTOF) enables the detecting up to 135 parameters on single cell, the emergence of which is an opportunity for proteomics research. We first tried to apply CyTOF technique to gastric cancer cells. We verified applicability of CyTOF in gastric cancer cells, and analyzed the responses of seventeen proteins to chemoradiotherapy in human gastric cancer AGS cells. To analyze the high dimensional CyTOF data, we used two statistical and visualization tools including viSNE and Citrus. Two specific clusters were found which had differences in protein expression profiles. CyTOF technology is proved feasibility and value at single cell level of gastric cancer.
化疗和放疗是治疗胃癌的主要辅助疗法，治疗效果存在个体差异，但具体机制尚不清楚。CyTOF 2 质谱流式细胞仪（CyTOF）能够在单个细胞上检测多达 135 个参数，它的出现为蛋白质组学研究提供了机会。我们首次尝试将 CyTOF 技术应用于胃癌细胞。我们验证了 CyTOF 在胃癌细胞中的适用性，并分析了 17 种蛋白对人胃癌 AGS 细胞放化疗的反应。为了分析高维 CyTOF 数据，我们使用了两种统计和可视化工具，包括 viSNE 和 Citrus。发现了两个具有不同蛋白表达谱的特定聚类。CyTOF 技术在胃癌的单细胞水平上被证明具有可行性和价值。
