Zhang Ting, Warden Antony R, Li Yiyang, Ding Xianting
State Key laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Clin Transl Med. 2020 Oct;10(6):e206. doi: 10.1002/ctm2.206.
Recently emerged mass cytometry (cytometry by time-of-flight [CyTOF]) technology permits the identification and quantification of inherently diverse cellular systems, and the simultaneous measurement of functional attributes at the single-cell resolution. By virtue of its multiplex ability with limited need for compensation, CyTOF has led a critical role in immunological research fields. Here, we present an overview of CyTOF, including the introduction of CyTOF principle and advantages that make it a standalone tool in deciphering immune mysteries. We then discuss the functional assays, introduce the bioinformatics to interpret the data yield via CyTOF, and depict the emerging clinical and research applications of CyTOF technology in sketching immune landscape in a wide variety of diseases.
最近出现的质谱流式细胞术（飞行时间流式细胞术[CyTOF]）技术能够识别和定量本质上多样化的细胞系统，并在单细胞分辨率下同时测量功能属性。凭借其多重检测能力且补偿需求有限，CyTOF在免疫学研究领域发挥了关键作用。在此，我们对CyTOF进行概述，包括介绍CyTOF的原理及其优势，这些优势使其成为破解免疫奥秘的独立工具。然后，我们讨论功能测定方法，介绍用于解读通过CyTOF产生的数据的生物信息学，并描述CyTOF技术在描绘多种疾病免疫图谱方面新兴的临床和研究应用。
