Shishodia Sonia Kumari, Tiwari Shraddha, Shankar Jata
Genomic Laboratory, Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, India.
Mycology. 2019 Feb 6;10(3):151-165. doi: 10.1080/21501203.2019.1574927. eCollection 2019.
species contain pathogenic and opportunistic fungal pathogens which have the potential to cause mycosis (invasive aspergillosis) in humans. The existing antifungal drugs have limitation largely due to the development of drug-resistant isolates. To gain insight into the mechanism of action and antifungal drug resistance in species including biofilm formation, we have reviewed protein data of species during interaction with antifungals drugs (polynes, azoles and echinocandin) and phytochemicals (artemisinin, coumarin and quercetin). Our analyses provided a list of proteins (72 proteins) that were abundant during interaction with different antifungal agents. On the other hand, there are 26 proteins, expression level of which is affected by more than two antifungal agents, suggesting the more general response to the stress induced by the antifungal agents. Our analysis showed enzymes from cell wall remodelling, oxidative stress response and energy metabolism are the responsible factors for providing resistance against antifungal drugs in species and could be explored further in clinical isolates. Also, these findings have clinical importance since the effect of drug targeting different proteins can be potentiated by combination therapy. We have also discussed the opportunities ahead to study the functional role of proteins from environmental and clinical isolates of during its interaction with the antifungal drugs. : IPA: invasive pulmonary aspergillosis; IA: invasive aspergillosis; AmB: Amphotericin B; CAS: Caspofungin; VRC: Voriconazole; ITC: Itraconazole; POS: Posaconazole; ART: Artemisinin; QRT: Quercetin; CMR: Coumarin; MIC: minimal inhibitory concentration.
某些物种含有致病性和机会性真菌病原体，它们有可能在人类中引起真菌病（侵袭性曲霉病）。现有的抗真菌药物存在局限性，这主要是由于耐药菌株的出现。为了深入了解包括生物膜形成在内的某些物种的作用机制和抗真菌药物耐药性，我们回顾了某些物种在与抗真菌药物（多烯类、唑类和棘白菌素）以及植物化学物质（青蒿素、香豆素和槲皮素）相互作用期间的蛋白质数据。我们的分析提供了一份在与不同抗真菌剂相互作用期间丰富的72种蛋白质清单。另一方面，有26种蛋白质，其表达水平受到两种以上抗真菌剂的影响，这表明对由抗真菌剂诱导的应激有更普遍的反应。我们的分析表明，细胞壁重塑、氧化应激反应和能量代谢中的酶是某些物种中提供抗真菌药物耐药性的关键因素，并且可以在临床分离株中进一步探索。此外，这些发现具有临床重要性，因为联合治疗可以增强针对不同蛋白质的药物效果。我们还讨论了未来研究某些物种的环境和临床分离株中的蛋白质在与抗真菌药物相互作用期间的功能作用的机会。：IPA：侵袭性肺曲霉病；IA：侵袭性曲霉病；AmB：两性霉素B；CAS：卡泊芬净；VRC：伏立康唑；ITC：伊曲康唑；POS：泊沙康唑；ART：青蒿素；QRT：槲皮素；CMR：香豆素；MIC：最低抑菌浓度。
