Department of Chemistry, School of Engineering Science in Chemistry, Biotechnology and Health , KTH Royal Institute of Technology , Teknikringen 30 , SE-100 44 Stockholm , Sweden.
Department of Clinical Science, Intervention and Technology, Karolinska Institutet, and Division of Obstetrics and Gynecology , Karolinska Universitetssjukhuset , SE-141 86 Stockholm , Sweden.
ACS Sens. 2019 Sep 27;4(9):2524-2535. doi: 10.1021/acssensors.9b01322. Epub 2019 Sep 6.
We present the most complete study to date comprising in vitro cytotoxicity tests of ion-selective membranes (ISMs) in terms of cell viability, proliferation, and adhesion assays with human dermal fibroblasts. ISMs were prepared with different types of plasticizers and ionophores to be tested in combination with assays that focus on the medium-term and long-term leaching of compounds. Furthermore, the ISMs were prepared in different configurations considering (i) inner-filling solution-type electrodes, (ii) all-solid-state electrodes based on a conventional drop-cast of the membrane, (iii) peeling after the preparation of a wearable sensor, and (iv) detachment from a microneedle-based sensor, thus covering a wide range of membrane shapes. One of the aims of this study, other than the demonstration of the biocompatibility of various ISMs and materials tested herein, is to create an awareness in the scientific community surrounding the need to perform biocompatibility assays during the very first steps of any sensor development with an intended biomedical application. This will foster meeting the requirements for subsequent on-body application of the sensor and avoiding further problems during massive validations toward the final in vivo use and commercialization of such devices.
我们展示了迄今为止最完整的研究，包括对离子选择性膜（ISMs）的体外细胞毒性测试，涉及细胞活力、增殖和人真皮成纤维细胞粘附测定。用不同类型的增塑剂和离子载体制备 ISMs，以与重点关注化合物中短期和长期浸出的测定相结合进行测试。此外，考虑到 (i) 内填充溶液型电极、(ii) 基于常规膜滴铸的全固态电极、(iii) 可穿戴传感器制备后的剥离和 (iv) 基于微针的传感器的分离，我们以不同的配置制备了 ISMs，从而涵盖了广泛的膜形状。本研究的目的除了证明各种 ISMs 和本文测试的材料的生物相容性外，还旨在引起科学界的关注，即需要在任何具有预期生物医学应用的传感器开发的最初步骤中进行生物相容性测定。这将有助于满足传感器随后在体内应用的要求，并避免在对这些设备进行最终体内使用和商业化的大规模验证过程中出现进一步的问题。
