Federal University of Santa Maria , Department of Chemistry , 97.105-900 , Santa Maria , RS , Brazil.
University of São Paulo , Center of Nuclear Energy in Agriculture , 13.416-000 , Piracicaba , SP , Brazil.
Environ Sci Technol. 2019 Sep 17;53(18):10827-10834. doi: 10.1021/acs.est.9b02868. Epub 2019 Sep 9.
The production and use of nanoparticles (NPs) in different fields increased in the last years. However, some NPs have toxicological properties, making these materials potential emerging pollutants. Therefore, it is important to investigate the uptake, transformation, translocation, and deposition of NPs in plants. In this work, laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) and micro X-ray fluorescence (μ-XRF) were used to investigate the uptake and translocation of LaO NPs to stem and leaves of (Spreng.) Pedersen after in vitro cultivation of plants in the presence of 400 mg L of LaO NPs. By using LA-ICP-MS and μ-XRF, image of the spatial distribution of La in the leaves was obtained, where higher concentration of La was observed in the main veins. Differences in the signal profile of La in leaves of plants cultivated in the presence of bulk LaO (b-LaO) and LaO NPs were observed. Sharp peaks of La indicated that NPs were transported to the stems and leaves of plants treated with LaO NPs. Both LA-ICP-MS and μ-XRF techniques have shown to be useful for detecting NPs in plants, but LA-ICP-MS is more sensitive than μ-XRF and allowed better detection and visualization of La distribution in the whole leaf.
近年来，纳米粒子（NPs）在不同领域的生产和使用有所增加。然而，一些 NPs 具有毒理学特性，使这些材料成为潜在的新兴污染物。因此，研究 NPs 在植物中的摄取、转化、迁移和沉积非常重要。在这项工作中，激光烧蚀-电感耦合等离子体质谱（LA-ICP-MS）和微 X 射线荧光（μ-XRF）被用于研究 LaO NPs 在体外培养的植物中存在 400mg/L LaO NPs 时向茎和叶中的摄取和转运。通过使用 LA-ICP-MS 和 μ-XRF，获得了 La 在叶片中空间分布的图像，其中在主叶脉中观察到更高浓度的 La。在含有块状 LaO（b-LaO）和 LaO NPs 的植物培养中观察到 La 信号谱的差异。La 的尖锐峰表明 NPs 被运输到用 LaO NPs 处理的植物的茎和叶中。LA-ICP-MS 和 μ-XRF 技术都已被证明可用于检测植物中的 NPs，但 LA-ICP-MS 比 μ-XRF 更灵敏，可更好地检测和可视化整个叶片中的 La 分布。
