Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.
Zoonoses Public Health. 2019 Dec;66(8):909-917. doi: 10.1111/zph.12641. Epub 2019 Aug 26.
Chikungunya virus (CHIKV) is a mosquito-borne pathogen that causes CHIKV fever. Definitive diagnosis is crucial for patients experiencing symptoms similar to other arboviral diseases because they can vary in clinical consequences. An increasing number of patients experience long-term rheumatic effects of CHIKV infection, but these cases may not be optimally detected by molecular assays and anti-CHIKV IgM ELISAs (M-ELISAs) used for confirmation and screening, respectively. The subsequent confirmatory serological test, the plaque reduction neutralization test (PRNT), is laborious and time-consuming. In this study, we evaluated a new diagnostic algorithm in which the M-ELISA is conducted in parallel with an anti-CHIKV IgG ELISA (G-ELISA) and observed that the Euroimmun M-ELISA combined with the Euroimmun G-ELISA or the Abcam G-ELISA exhibited excellent sensitivity and specificity for CHIKV. The combinations demonstrated perfect and near perfect inter-rater agreement with the PRNT, respectively, suggesting their potential to be used as alternatives to the confirmatory serological PRNT assay for CHIKV.
基孔肯雅热病毒（CHIKV）是一种通过蚊子传播的病原体，可引起基孔肯雅热。对于出现类似于其他虫媒病毒病的症状的患者，明确诊断至关重要，因为它们在临床后果上可能有所不同。越来越多的患者经历基孔肯雅病毒感染的长期风湿影响，但这些病例可能无法通过用于确认和筛查的分子检测和抗基孔肯雅病毒 IgM ELISA（M-ELISA）得到最佳检测。随后的确认血清学检测，即蚀斑减少中和试验（PRNT），既繁琐又耗时。在这项研究中，我们评估了一种新的诊断算法，其中 M-ELISA 与抗基孔肯雅病毒 IgG ELISA（G-ELISA）并行进行，并观察到 Euroimmun M-ELISA 与 Euroimmun G-ELISA 或 Abcam G-ELISA 的组合对基孔肯雅病毒具有优异的敏感性和特异性。这些组合与 PRNT 的观察者间一致性分别为完美和近乎完美，这表明它们有潜力替代基孔肯雅病毒的确认血清学 PRNT 检测。
