苏云金芽孢杆菌以色列亚种双功能基因标记质粒的构建
Construction of a bifunctional genetically labelled plasmid for Bacillus thuringiensis subsp. israelensis.
作者信息
Miteva V I, Grigorova R T
机构信息
Department of Genetics, Bulgarian Academy of Sciences, Sofia.
出版信息
Arch Microbiol. 1988;150(5):496-8. doi: 10.1007/BF00422293.
PMID:3144956
Abstract
A small cryptic plasmid of Bacillus thuringiensis subsp. israelensis was labelled in vitro with two genetic markers. One of the recombinant plasmids was mapped and transformed in Escherichia coli, Bacillus subtilis and Bacillus thuringiensis. This and similar shuttle plasmids could be very useful as vectors for the investigation of the toxin genes in their own host.
摘要
苏云金芽孢杆菌以色列亚种的一个小型隐蔽质粒在体外被两个遗传标记物标记。其中一个重组质粒被进行了图谱分析，并转化到大肠杆菌、枯草芽孢杆菌和苏云金芽孢杆菌中。这种及类似的穿梭质粒作为载体对于在其自身宿主中研究毒素基因可能非常有用。
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