枯草芽孢杆菌中双功能质粒载体的分子克隆：分离一株对在大肠杆菌中繁殖的嵌合质粒DNA具有增强转化能力的枯草芽孢杆菌自发突变体。
Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis: isolation of a spontaneous mutant of Bacillus subtilis with enhanced transformability for Escherichia coli-propagated chimeric plasmid DNA.
作者信息
Ostroff G R, Pène J J
出版信息
J Bacteriol. 1983 Nov;156(2):934-6. doi: 10.1128/jb.156.2.934-936.1983.
Abstract
Hybrid plasmid DNA cloned in Escherichia coli undergoes deletions when returned to competent Bacillus subtilis, even in defined restriction and modification mutants of strain 168. We have isolated a mutant of B. subtilis MI112 which is stably transformed at high frequency by chimeric plasmid DNA propagated in E. coli.
摘要
克隆于大肠杆菌中的杂种质粒DNA，即便导入枯草芽孢杆菌168株特定的限制与修饰突变体中，当再导入感受态枯草芽孢杆菌时仍会发生缺失。我们已分离出枯草芽孢杆菌MI112的一个突变体，它能被在大肠杆菌中增殖的嵌合质粒DNA以高频稳定转化。
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本文引用的文献
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