灰色链霉菌富含细胞色素P-450的提取物对外源化合物的氧化作用。
Xenobiotic oxidation by cytochrome P-450-enriched extracts of Streptomyces griseus.
作者信息
Trower M K, Sariaslani F S, Kitson F G
机构信息
E.I. Du Pont de Nemours & Co. (Inc.), Central Research and Development Department, Wilmington, DE 19880-0228.
出版信息
Biochem Biophys Res Commun. 1988 Dec 30;157(3):1417-22. doi: 10.1016/s0006-291x(88)81033-7.
Crude extracts of Streptomyces griseus grown on soybean flour-enriched medium contain high levels of cytochrome P-450. The cytochrome P-450-enriched fractions, obtained by ammonium sulfate fractionation (30-50% saturation), catalyze the NADPH-dependent oxidation of a variety of xenobiotics when complemented with both spinach ferredoxin:NADP+ oxidoreductase and spinach ferredoxin. Reactions observed are aromatic, benzylic and alicyclic hydroxylations, O-dealkylation, non-aromatic double bond epoxidation, N-oxidation and N-acetylation.
在富含大豆粉的培养基上生长的灰色链霉菌粗提物含有高水平的细胞色素P-450。通过硫酸铵分级分离（30-50%饱和度）获得的富含细胞色素P-450的组分，在与菠菜铁氧还蛋白：NADP+氧化还原酶和菠菜铁氧还蛋白互补时，催化多种外源化合物的NADPH依赖性氧化。观察到的反应包括芳香族、苄基和脂环族羟基化、O-脱烷基化、非芳香族双键环氧化、N-氧化和N-乙酰化。
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