Vazeille M C, Rosen L
Pacific Biomedical Research Center, University of Hawaii, Honolulu 96822.
Ann Inst Pasteur Virol. 1988 Apr-Jun;139(2):175-83. doi: 10.1016/s0769-2617(88)80015-7.
Antisera were prepared in rabbits against two strains of sigma virus, from France and Hawaii respectively, using purified virus from cell cultures. All antisera had the same titre by immunofluorescence, 1/400 or 1/800, whether tested against the homologous or the heterologous virus. An antiserum prepared with the French strain neutralized the Hawaiian virus at a dilution of 1/800 both in vitro and in vivo. The Hawaiian virus was not neutralized to a significant extent by reference mouse ascitic fluids for 26 different vertebrate rhabdoviruses.
用从细胞培养物中纯化的病毒,分别针对来自法国和夏威夷的两株西格玛病毒,在兔体内制备抗血清。通过免疫荧光检测,所有抗血清的效价相同,均为1/400或1/800,无论检测的是同源病毒还是异源病毒。用法国毒株制备的抗血清在体外和体内均能以1/800的稀释度中和夏威夷病毒。对于26种不同的脊椎动物弹状病毒,夏威夷病毒未被参考小鼠腹水显著中和。