Cybinski D H, Davis S S, Zakrzewski H
CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Indooroopilly, Queensland, Australia.
Arch Virol. 1992;124(3-4):211-24. doi: 10.1007/BF01309803.
Glycoprotein-specific monoclonal antibodies (MAbs) were used to select escape mutants of bovine ephemeral fever (BEF) virus to determine the escape frequency for different epitopes and to construct an epitope map. At least six antigenic sites were detected by this method and escape frequencies between 10(-2) and 10(-8) were recorded. One new non-conformational site was defined by a MAb, 5A5, which neutralized Berrimah and Kimberley viruses as well as three BEF virus strains. Batch to batch variation was detected in the BB7721 strain of BEF virus when tested for MAb neutralization. Eighteen strains of BEF virus, isolated from blood and insects from a variety of locations in Australia over a period of 33 years, were examined using MAbs and at least one epitope could not be detected in strains isolated since 1975. Implications for vaccine development are discussed.
使用糖蛋白特异性单克隆抗体（MAb）来筛选牛暂时热（BEF）病毒的逃逸突变体，以确定不同表位的逃逸频率并构建表位图谱。通过该方法检测到至少六个抗原位点，记录的逃逸频率在10^(-2)至10^(-8)之间。一种单克隆抗体5A5定义了一个新的非构象位点，该抗体可中和贝里马病毒、金伯利病毒以及三株BEF病毒毒株。在对BEF病毒的BB7721毒株进行单克隆抗体中和试验时，检测到了批次间差异。使用单克隆抗体对从澳大利亚不同地点在33年期间采集的血液和昆虫中分离出的18株BEF病毒进行了检测，自1975年以来分离出的毒株中至少有一个表位未被检测到。文中讨论了其对疫苗开发的影响。
