Silesian University of Technology, Faculty of Power and Environmental Engineering, Environmental Biotechnology Department, Akademicka 2, 44-100, Gliwice, Poland.
Silesian University of Technology, Faculty of Power and Environmental Engineering, Environmental Biotechnology Department, Akademicka 2, 44-100, Gliwice, Poland.
Chemosphere. 2020 Jan;238:124597. doi: 10.1016/j.chemosphere.2019.124597. Epub 2019 Aug 15.
In this study, we focused on the proportion of particular bacterial groups and changes in microbial community structure in relation to the anammox process parameters and the feeding medium strategy in the Sequencing Batch Reactor (SBR). In order to present an insight into the microbial dynamics while feeding medium shift from synthetic wastewater to landfill leachate, fluorescent in situ hybridization (FISH), Real Time PCR, PCR - DGGE (Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis) and Reverse Transcription PCR-DGGE analysis were used. Feeding medium change has the strongest impact on relative abundance of denitrifiers and representatives of Planctomycetes. The relative abundancy of specific genes for all investigated nitrogen removal bacterial groups dropped after landfill leachate implementation. However, anammox consortium were able to adapt to the new reactor operating conditions and time for adaptation was estimated at the level of 90 days.
在这项研究中，我们专注于特定细菌群体的比例以及微生物群落结构的变化与序批式反应器（SBR）中的厌氧氨氧化工艺参数和进料介质策略的关系。为了深入了解从合成废水到垃圾渗滤液进料介质转变过程中的微生物动态，我们使用了荧光原位杂交（FISH）、实时 PCR、PCR-DGGE（聚合酶链反应-变性梯度凝胶电泳）和反转录 PCR-DGGE 分析技术。进料介质的变化对反硝化菌和浮霉菌代表物的相对丰度有最强的影响。在实施垃圾渗滤液后，所有被调查的氮去除细菌群体的特定基因的相对丰度都下降了。然而，厌氧氨氧化菌能够适应新的反应器操作条件，适应时间估计在 90 天的水平。
