Méchali M, Gusse M, Vriz S, Taylor M, Andéol Y, Moreau J, Hourdry J, Leibovici M, Brulfert A, Almouzni G
Institut Jacques Monod, Paris, France.
Biochimie. 1988 Jul;70(7):895-8. doi: 10.1016/0300-9084(88)90230-1.
The role of proto-oncogenes in embryonic development was investigated using one of the most characterized vertebrates, the amphibian Xenopus laevis. Genes which belong to the major proto-oncogene families have been detected in Xenopus genome. The developmental control of the myc gene was assayed using a characterized Xenopus myc probe and specific antibodies. The myc gene is highly expressed as a stable maternal mRNA in oocyte, and an unfertilized egg contains 5 X 10(5)-fold the myc RNA content of a proliferative somatic cell. The myc RNA store is evenly distributed in the oocyte and the egg. Fertilization triggers a post-transcriptional control of the gene and the RNA store is progressively degraded to a constitutive value of 10 to 30 myc RNA copies registered per gastrula embryonic cell. The 62K myc protein is accumulated late in oogenesis. This uncoupling of myc expression and cell proliferation appears as a specific developmental regulation of the myc gene, adapted to the series of rapid cell cleavages occurring after fertilization.
利用最具代表性的脊椎动物之一——两栖动物非洲爪蟾，对原癌基因在胚胎发育中的作用进行了研究。在非洲爪蟾基因组中已检测到属于主要原癌基因家族的基因。使用经过鉴定的非洲爪蟾myc探针和特异性抗体，对myc基因的发育调控进行了分析。myc基因在卵母细胞中作为一种稳定的母源mRNA高度表达，未受精卵中myc RNA的含量是增殖性体细胞的5×10⁵倍。myc RNA库在卵母细胞和卵中均匀分布。受精引发该基因的转录后调控，RNA库逐渐降解至每个原肠胚胚胎细胞记录到的10至30个myc RNA拷贝的组成型值。62K myc蛋白在卵子发生后期积累。myc表达与细胞增殖的这种解偶联表现为myc基因的一种特定发育调控，适用于受精后发生的一系列快速细胞分裂。
