Hourdry J, Brulfert A, Gusse M, Schoevaert D, Taylor M V, Mechali M
Institut Jacques Monod, Paris, France.
Development. 1988 Dec;104(4):631-41. doi: 10.1242/dev.104.4.631.
The expression of the proto-oncogene c-myc during oogenesis and embryonic development was followed by in situ hybridization using a cytological protocol adapted to amphibian embryos. The c-myc RNA was highly expressed in the cytoplasm of young oocytes and was further diluted during oocyte growth without specific localization. From the neurula stage on, new myc transcripts were detected and the whole embryo appeared positive with antisense myc RNA probes relative to control sense RNA probes. In addition, a spatial localization of high levels of the transcript was also observed in specific areas of the developing embryo, including the epidermis, gill buds, optic vesicles and lens placodes. These observations might indicate a specific role of the c-myc gene during the differentiation of these tissues. Alternatively, this high level of myc expression might prevent such tissues from entering into terminal differentiation during the growth of the embryo.
利用适用于两栖类胚胎的细胞学方案，通过原位杂交追踪原癌基因c-myc在卵子发生和胚胎发育过程中的表达情况。c-myc RNA在年轻卵母细胞的细胞质中高度表达，并在卵母细胞生长过程中进一步稀释，且无特异性定位。从神经胚阶段开始，检测到新的myc转录本，相对于对照正义RNA探针，用反义myc RNA探针检测时整个胚胎呈阳性。此外，在发育中胚胎的特定区域，包括表皮、鳃芽、视泡和晶状体基板，也观察到转录本的高水平空间定位。这些观察结果可能表明c-myc基因在这些组织的分化过程中具有特定作用。或者，这种高水平的myc表达可能会阻止这些组织在胚胎生长过程中进入终末分化。
