McGroarty J A, Reid G
Urology Research, Toronto General Hospital, Ont., Canada.
Can J Microbiol. 1988 Aug;34(8):974-8. doi: 10.1139/m88-171.
Recent studies have shown that certain lactobacilli strains have the ability to interfere with the adherence and growth of uropathogenic bacteria. This interaction is believed to be important in the maintenance of a normal urogenital flora and in the prevention of infection in females. In the present study, Lactobacillus casei ssp. rhamnosus GR-1 and Lactobacillus acidophilus 76 were found to exert an inhibitory effect on pyelonephritogenic mutant Escherichia coli Hu 734 and E. coli ATCC 25922. The bioactivity of the inhibitor produced by strain GR-1 was retained under pH buffered conditions and was bactericidal. The bioactive substance was heat labile, not precipitated by up to 80% ammonium sulphate, and extractable in chloroform. The data indicated that the inhibitor is not lactic acid or hydrogen peroxide and has a molecular weight greater than 12,000-14,000. Human urine supported production of the inhibitor and reduced and delayed outgrowth of the E. coli. The ability of L. casei GR-1 and possibly other lactobacilli strains to produce inhibitors of uropathogenic bacteria may have clinical importance and significance in the microbial ecology of the urogenital tract.
最近的研究表明，某些乳酸杆菌菌株有能力干扰尿路致病性细菌的黏附和生长。这种相互作用被认为在维持正常泌尿生殖系统菌群以及预防女性感染方面很重要。在本研究中，发现干酪乳杆菌鼠李糖亚种GR-1和嗜酸乳杆菌76对致肾盂肾炎突变型大肠杆菌Hu 734和大肠杆菌ATCC 25922有抑制作用。GR-1菌株产生的抑制剂的生物活性在pH缓冲条件下得以保留，且具有杀菌作用。该生物活性物质对热不稳定，不被高达80%的硫酸铵沉淀，可在氯仿中提取。数据表明该抑制剂不是乳酸或过氧化氢，分子量大于12,000 - 14,000。人尿支持该抑制剂的产生，并减少和延迟了大肠杆菌的生长。干酪乳杆菌GR-1以及可能其他乳酸杆菌菌株产生尿路致病性细菌抑制剂的能力在泌尿生殖道的微生物生态学中可能具有临床重要性和意义。
