Watanabe T, Kawai Y, Mutai M
Microbiol Immunol. 1980;24(1):21-9. doi: 10.1111/j.1348-0421.1980.tb00558.x.
To learn the biochemical mechanisms controlling the distribution of indigenous lactobacilli in the gastrointestinal tracts of rats, the effect of pH and stomach and cecal contents on lactobacillus distribution was investigated in vitro with a mixed culture of three lactobacillus strains isolated from the rat intestine. The pH of the growth medium affected the growth of lactobacilli strongly, irrespective of the lumenal contents. Lactobacillus fermentum outnumbered L. acidophilus and L. murini at low pH (PH 4.5; average pH of stomach contents of conventional rats) but at near neutral pH (pH 6.5; average pH of cecal contents of conventional rats), the growth of L. murini was predominant with all strains. More lactic acid was formed by lactobacilli in medium consisting of stomach contents than in cecal contents medium. L. murini grew in the nondialyzable fraction of the stomach contents and L. fermentum grew in the dialyzable fraction, but L. acidophilus did not grow in either fraction. L. murini grew in the nondialyzable fraction treated with hyaluronidase. In contrast, the nondialyzable fraction treated with pronase or chondroitinase did not allow L. murini to grow at all.
为了解控制大鼠胃肠道中本土乳酸菌分布的生化机制，利用从大鼠肠道分离出的三株乳酸菌混合培养物，在体外研究了pH值以及胃和盲肠内容物对乳酸菌分布的影响。无论管腔内容物如何，生长培养基的pH值都对乳酸菌的生长有强烈影响。在低pH值（pH 4.5；常规大鼠胃内容物的平均pH值）下，发酵乳杆菌的数量超过嗜酸乳杆菌和鼠乳杆菌，但在接近中性的pH值（pH 6.5；常规大鼠盲肠内容物的平均pH值）下，所有菌株中鼠乳杆菌的生长占主导地位。与盲肠内容物培养基相比，乳酸菌在由胃内容物组成的培养基中产生的乳酸更多。鼠乳杆菌在胃内容物的不可透析部分生长，发酵乳杆菌在可透析部分生长，但嗜酸乳杆菌在这两个部分均不生长。鼠乳杆菌在经透明质酸酶处理的不可透析部分中生长。相反，经链霉蛋白酶或软骨素酶处理的不可透析部分完全不允许鼠乳杆菌生长。
