Department of Biology, Faculty of Science, Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai, 50202, Thailand.
Department of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50202, Thailand.
In Vitro Cell Dev Biol Anim. 2019 Oct;55(9):723-732. doi: 10.1007/s11626-019-00393-w. Epub 2019 Aug 26.
Several species of the Annonaceae plants have been used as complementary medicine for cancer-associated illnesses in some ethnic groups of northern Thailand. This study investigated the cytotoxic and cytostatic activity of methanolic extracts derived from the stems of these plants, including Uvaria longipes (Craib) L.L.Zhou, Y.C.F.Su & R.M.K.Saunders, Artabotrys burmanicus A.DC, Marsypopetalum modestum (Pierre) B.Xue & R.M.K.Saunders, and Dasymaschalon sp. Cell death induction of seven human cancer cell lines and cell cycle analyses were assessed by Annexin V and/or propidium iodide (PI) staining and analyzed by flow cytometry. Treatment of cancer cell lines with the extract of four Annonaceae plants resulted in various cytotoxic activities depending on cell type. The extract of U. longipes exhibited the highest cytotoxic activity capable of inducing cell death of several cancer cell lines, particularly against hepatocellular carcinoma cell lines (HepG2 and Hep3B). This extract was capable of inducing cell cycle arrest at the SubG1 phase. Phytochemical screening of all the extracts revealed the presence of alkaloids, sterols, tannins, anthraquinone glycoside, coumarin, and flavonoids. Determination of active compounds by high-performance liquid chromatography standards revealed bullatacin and asiminecin in all the extracts. The extract of Annonaceae stem or its compounds may provide an opportunity for the development of new therapies against cancer.
几种番荔枝科植物已被用于泰国北部一些少数民族群体的癌症相关疾病的辅助治疗。本研究调查了这些植物茎的甲醇提取物的细胞毒性和细胞抑制活性，包括 Uvaria longipes (Craib) L.L.Zhou, Y.C.F.Su & R.M.K.Saunders、Artabotrys burmanicus A.DC、Marsypopetalum modestum (Pierre) B.Xue & R.M.K.Saunders 和 Dasymaschalon sp. 通过 Annexin V 和/或碘化丙啶（PI）染色评估了七种人癌细胞系的细胞死亡诱导和细胞周期分析，并通过流式细胞术进行分析。四种番荔枝科植物提取物处理癌细胞系会根据细胞类型产生不同的细胞毒性活性。U. longipes 提取物表现出最高的细胞毒性活性，能够诱导几种癌细胞系（特别是肝癌细胞系 HepG2 和 Hep3B）死亡。该提取物能够诱导细胞周期停滞在 SubG1 期。所有提取物的植物化学筛选显示存在生物碱、甾醇、单宁、蒽醌糖苷、香豆素和类黄酮。高效液相色谱标准品测定活性化合物的结果表明，所有提取物中均存在蝙蝠葛苏林碱和阿西米宁。番荔枝科植物茎或其化合物的提取物可能为开发针对癌症的新疗法提供机会。
