Department of Chemistry and the Center for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Mahidol University, Bangkok, Thailand.
Fitoterapia. 2011 Oct;82(7):964-8. doi: 10.1016/j.fitote.2011.05.012. Epub 2011 May 27.
Bioassay-guided fractionation of the cytotoxic ethyl acetate extract from the stems of Dasymaschalon blumei (Annonaceae) led to the isolation of four aristololactam alkaloids, including the hitherto unknown 3,5-dihydroxy-2,4-dimethoxyaristolactam (1), as well as the three known compounds, aristolactam BI, goniopedaline, and griffithinam. Additionally, the cytotoxic extract from the combined leaves and twigs of the same plant yielded three known oxoaporphine alkaloids, oxodiscoguattine, dicentrinone, and duguevalline. The structures of aristolactams and oxoaporphine alkaloids were elucidated on the basis of spectroscopic methods. All isolates were evaluated for cytotoxicity against a panel of mammalian cancer cell lines and a noncancerous human embryonic kidney cell Hek 293.
生物测定指导下，从野八角（八角科）茎的细胞毒乙酸乙酯提取物中分离得到四种石松生物碱，包括迄今未知的 3,5-二羟基-2,4-二甲氧基石松生物碱（1），以及三种已知化合物，石松 BI、戈尼帕定和格里菲斯胺。此外，从同一植物的叶和小枝合并提取物中得到三种已知的氧化阿朴菲生物碱，氧化 discoguattine、二氢 discoguanthine 和 duguevalline。根据光谱方法阐明了石松生物碱和氧化阿朴菲生物碱的结构。所有分离物均针对一组哺乳动物癌细胞系和非癌细胞系人胚肾细胞 Hek 293 进行了细胞毒性评估。
