Roellig Dawn M, Xiao Lihua
Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Methods Mol Biol. 2020;2052:103-116. doi: 10.1007/978-1-4939-9748-0_7.
Cryptosporidium genotyping has made significant contributions to the species structure and population genetics of Cryptosporidium spp. In addition, the standardized method has shown utility in epidemiologic investigations identifying case linkages and contamination sources that could not be determined with traditional epidemiologic tools. The standardized method for Cryptosporidium genotyping from stool specimens described here comprises multiple individual protocols to amplify and sequence regions of the SSU rRNA and gp60 genes of Cryptosporidium.
隐孢子虫基因分型对隐孢子虫属的物种结构和群体遗传学做出了重大贡献。此外，该标准化方法在流行病学调查中显示出了实用价值，可识别传统流行病学工具无法确定的病例关联和污染源。本文所述的从粪便标本中进行隐孢子虫基因分型的标准化方法包括多个单独的方案，用于扩增和测序隐孢子虫的小亚基核糖体RNA（SSU rRNA）和gp60基因区域。
