通过qRT-PCR对体外抗微小隐孢子虫生长的药物进行高通量筛选
High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR.
作者信息
Zhang Haili, Zhu Guan
机构信息
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA.
出版信息
Methods Mol Biol. 2020;2052:319-334. doi: 10.1007/978-1-4939-9748-0_18.
An effective method to quantify the parasite loads is the key to the evaluation of anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening (HTS) of drugs against Cryptosporidium parvum in vitro was impractical by the labor-intensive traditional assays. Here we describe a simplified quantitative RT-PCR assay suitable for HTS of compounds and for evaluating drug efficacy against the growth of C. parvum in vitro.
定量寄生虫负荷的有效方法是体外评估抗隐孢子虫药物疗效的关键。然而，通过劳动强度大的传统检测方法对微小隐孢子虫进行体外高通量药物筛选是不切实际的。在此，我们描述了一种简化的定量逆转录聚合酶链反应检测方法，该方法适用于化合物的高通量筛选以及评估药物对体外微小隐孢子虫生长的疗效。
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