Pinon-Lataillade G, Vélez de la Calle J F, Viguier-Martinez M C, Garnier D H, Folliot R, Maas J, Jégou B
Commissariat à l'Energie Atomique, Laboratoire de Toxicologie des Transuraniens, Bruyères le Châtel, France.
Mol Cell Endocrinol. 1988 Jul;58(1):51-63. doi: 10.1016/0303-7207(88)90053-6.
The effects of continuous gamma-irradiation of adult rats at two low-dose rates (7 cGy and 12 cGy/day; up to a total dose of 9.1 Gy and 10.69 Gy 60Co gamma-ray, respectively) were investigated. Over a period of 3-131 days of irradiation, groups of experimental and control animals were killed. Body weight, testis, epididymis, prostate and seminal vesicle weights, the number of germ cells and Sertoli cells, tubular ultrastructure, epididymal and testicular levels of biologically active androgen-binding protein (ABP), and the plasma concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone were monitored. Irradiation had no effect on body weight, whereas testicular and epididymal weight began to decrease following 35 and 50 days of irradiation at 7 and 12 cGy, respectively. At 7 cGy the target cells of the gamma-rays were essentially A spermatogonia, whereas at 12 cGy A spermatogonia and preleptotene spermatocytes were primarily affected. This resulted in a progressive and sequential dose-related reduction in the number of pachytene spermatocytes, round spermatids and late spermatids (LS). Under both irradiation procedures the Sertoli cell number remained unchanged whereas partial (7 cGy) or no change (12 cGy) was seen at the Leydig cell level. Whatever the irradiation protocol, from the time LS numbers decreased, vacuolisation of the Sertoli cell cytoplasm progressively occurred, followed by thickening and folding of the peritubular tissue. Moreover, in parallel to the drop in the number of these germ cell types, ABP production fell whereas FSH levels rose. A highly significant positive correlation was found between LS numbers and these Sertoli cell parameters. This study supports our previous concept of a control of certain important aspects of Sertoli cell function by late spermatids in the adult rat.
研究了成年大鼠在两种低剂量率（分别为7 cGy和12 cGy/天；60Coγ射线的总剂量分别高达9.1 Gy和10.69 Gy）下连续γ照射的影响。在3至131天的照射期间，处死了实验组和对照组动物。监测了体重、睾丸、附睾、前列腺和精囊重量、生殖细胞和支持细胞数量、管状超微结构、附睾和睾丸中生物活性雄激素结合蛋白（ABP）水平以及促卵泡激素（FSH）、促黄体生成素（LH）和睾酮的血浆浓度。照射对体重没有影响，而在7 cGy和12 cGy照射35天和50天后，睾丸和附睾重量开始下降。在7 cGy时，γ射线的靶细胞主要是A型精原细胞，而在12 cGy时，A型精原细胞和前细线期精母细胞受到主要影响。这导致粗线期精母细胞、圆形精子细胞和晚期精子细胞（LS）数量逐渐且与剂量相关地减少。在两种照射程序下，支持细胞数量保持不变，而在睾丸间质细胞水平上观察到部分（7 cGy）或无变化（12 cGy）。无论照射方案如何，从LS数量减少时起，支持细胞细胞质逐渐出现空泡化，随后是管状周围组织增厚和折叠。此外，与这些生殖细胞类型数量的下降同时，ABP产生减少而FSH水平升高。在LS数量与这些支持细胞参数之间发现了高度显著的正相关。本研究支持了我们之前关于成年大鼠晚期精子细胞控制支持细胞功能某些重要方面的概念。
