Kamtchouing P, Pinon-Lataillade G, Papadopoulos V, Guillaumin J M, Bardos P, Maas J, Perreau C, Drosdowsky M A, Hochereau de Reviers M T, Carreau S
Laboratoire de Biochimie, UA CNRS 609, CHU Côte de Nâcre, Caen, France.
Reprod Nutr Dev (1980). 1988;28(4B):1009-17. doi: 10.1051/rnd:19880702.
Continuous low-dose gamma-irradiation of mature rats induced a progressive degeneration of the germ cells. Blood FSH increased by 127, 176 and 214%, respectively, after 55, 70 and 85 days of treatment when compared to FSH levels in control rats (8.50 +/- 0.60 ng/ml); conversely, serum LH and testosterone levels were unchanged. The Sertoli cell function was affected by the treatment from 70 days on, as attested by androgen binding protein (ABP) and transferrin secretions which diminished 35-40%. Serum ABP levels were not altered, whatever the duration of irradiation, even though epididymal ABP contents (as well as concentrations) diminished 34-60% when compared to those of the controls. Moreover, in purified Leydig cells, LH-stimulated intracellular cAMP levels, which were decreased by seminiferous tubule medium (STM) from control rats, were enhanced in presence of STM from treated animals. Testosterone output was stimulated 9-fold in presence of oLH and further increased (46-76%) from stages XIV-V by STM prepared from control and irradiated rats, respectively. After 85 days the STM effects on both cAMP and testosterone syntheses were zero. These results demonstrate a probable alteration of Sertoli cell function after irradiation, but also a role of the germ cells in the regulation of the synthesis of ABP, transferrin and Sertoli cell paracrine factors.
对成年大鼠进行连续低剂量γ射线照射会导致生殖细胞逐渐退化。与对照大鼠的促卵泡激素（FSH）水平（8.50±0.60 ng/ml）相比，在治疗55、70和85天后，血液中的FSH分别增加了127%、176%和214%；相反，血清促黄体生成素（LH）和睾酮水平没有变化。从70天起，支持细胞功能受到治疗影响，这通过雄激素结合蛋白（ABP）和转铁蛋白分泌减少35 - 40%得到证实。无论照射持续时间如何，血清ABP水平均未改变，尽管与对照组相比，附睾ABP含量（以及浓度）减少了34 - 60%。此外，在纯化的睾丸间质细胞中，来自对照大鼠的生精小管培养基（STM）会降低促黄体生成素刺激的细胞内cAMP水平，而来自经处理动物的STM存在时则会使其升高。在促黄体生成素（oLH）存在的情况下，睾酮产量被刺激增加9倍，并且分别由对照和照射大鼠制备的STM从第十四 - 十五阶段进一步增加（46 - 76%）。85天后，STM对cAMP和睾酮合成的影响均为零。这些结果表明照射后支持细胞功能可能发生改变，同时也表明生殖细胞在ABP、转铁蛋白和支持细胞旁分泌因子合成调节中的作用。
