Département de Sciences Biologiques, Institut de Recherche en Biologie Végétale, Université de Montréal, 4101 Sherbrooke Est, Montreal, QC, H1X 2B2, Canada.
Quebec Centre for Biodiversity Science, Montreal, QC, Canada.
Microb Biotechnol. 2019 Nov;12(6):1346-1358. doi: 10.1111/1751-7915.13473. Epub 2019 Aug 26.
Seaweeds have been used as a source of natural fertilizer and biostimulant in agriculture for centuries. However, their effects on soil and crop root microbiota remain unclear. Here, we used a commercially available Ascophyllum nodosum extract (ANE) to test its effect on bacterial and fungal communities of rhizospheric soils and roots of pepper and tomato plants in greenhouse trials. Two independent trials were conducted in a split-block design. We used amplicon sequencing targeting fungal ITS and bacterial 16S rRNA gene to determine microbial community structure changes. We find that productivity parameters of root, shoot and fruit biomass were positively and significantly influenced by the ANE amendment. In addition, a-diversity differed significantly between amended and control plants, but only in some of the experimental conditions. Species composition among sites (b-diversity) differed according to the amendment treatment in all four communities (fungal-root, fungal-soil, bacterial-root and bacterial-soil). Finally, we identified a number of candidate taxa most strongly correlated with crop yield increases. Further studies on isolation and characterization of these microbial taxa linked to the application of liquid seaweed extract may help to enhance crop yield in sustainable agro-ecosystems.
海藻在农业中作为天然肥料和生物刺激素的来源已经有几个世纪了。然而，它们对土壤和作物根际微生物区系的影响尚不清楚。在这里，我们使用一种市售的泡叶藻提取物（ANE），在温室试验中测试其对辣椒和番茄植株根际土壤和根系细菌和真菌群落的影响。我们进行了两个独立的裂区设计试验。我们使用靶向真菌 ITS 和细菌 16S rRNA 基因的扩增子测序来确定微生物群落结构的变化。我们发现，根、茎和果实生物量的生产力参数受到 ANE 处理的显著正向影响。此外，在一些实验条件下，处理和对照植物之间的α多样性差异显著。在所有四个群落（真菌-根、真菌-土壤、细菌-根和细菌-土壤）中，根据处理方式，站点间的物种组成（β多样性）也存在差异。最后，我们确定了一些与作物产量增加最密切相关的候选分类群。进一步研究这些与液体海藻提取物应用相关的微生物类群的分离和特征，可能有助于提高可持续农业生态系统中的作物产量。
