Zhong Xiaowu, Peng Yuanhong, Liao Hebin, Yao Chengjiao, Li Jiulong, Yang Qibin, He Yonglong, Qing Yufeng, Guo Xiaolan, Zhou Jingguo
Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
Translational Medicine Research Center, North Sichuan Medical College, Nanchong, Sichuan 637007, P.R. China.
Exp Ther Med. 2019 Sep;18(3):1967-1976. doi: 10.3892/etm.2019.7816. Epub 2019 Jul 26.
Gouty arthritis (GA) is the most common inflammatory and immune-associated disease, and its prevalence and incidence exhibit yearly increases. The aim of the present study was to analyse the expression profile variation of long non-coding RNAs (lncRNAs) in GA patients and to explore the role of lncRNAs in the pathogenesis of GA. The peripheral blood mononuclear cells of GA patients and of healthy controls (HCs) were used to detect for the differentially expressed lncRNAs by microarray. The functional annotations and classifications of the differentially expressed transcripts were predicted using Gene Ontology (GO) and pathway analysis. The results were then verified by reverse transcription-quantitative (RT-q)PCR. A total of 1,815 lncRNAs and 971 mRNAs with a >2-fold difference in the levels of expression in the GA patients compared with those in the HCs were identified. According to the GO functional enrichment analysis, the differentially expressed lncRNAs were accumulated in terms including protein binding, catalytic activity and molecular transducer activity. The pathways predicted to be involved were the tumor necrosis factor signaling pathway, osteoclast differentiation, NOD-like receptor signaling pathway and NF-κB signaling pathway. The expression of six lncRNAs was measured by RT-qPCR and the results were consistent with those of the microarrays. Among these lncRNAs, AJ227913 was the most differentially expressed lncRNA in GA patients vs. HCs. The expression of several lncRNAs was significantly changed in GA patients compared with that in HCs, which suggests that these lncRNAs with differential expression levels may have an important role in the development and progression of GA.
痛风性关节炎（GA）是最常见的炎症性和免疫相关疾病，其患病率和发病率逐年上升。本研究的目的是分析GA患者中长链非编码RNA（lncRNA）的表达谱变化，并探讨lncRNA在GA发病机制中的作用。采用GA患者和健康对照者（HCs）的外周血单个核细胞，通过微阵列检测差异表达的lncRNA。使用基因本体论（GO）和通路分析预测差异表达转录本的功能注释和分类。然后通过逆转录定量（RT-q）PCR验证结果。与HCs相比，共鉴定出1815个lncRNA和971个mRNA在GA患者中的表达水平差异>2倍。根据GO功能富集分析，差异表达的lncRNA在蛋白质结合、催化活性和分子转导活性等方面富集。预测涉及的通路有肿瘤坏死因子信号通路、破骨细胞分化、NOD样受体信号通路和NF-κB信号通路。通过RT-qPCR检测了6个lncRNA的表达，结果与微阵列结果一致。在这些lncRNA中，AJ227913是GA患者与HCs中差异表达最明显的lncRNA。与HCs相比，GA患者中几种lncRNA的表达发生了显著变化，这表明这些表达水平有差异的lncRNA可能在GA的发生和发展中起重要作用。
