Zhang Qifu, Sui Yana, Sui Xiaomei
Radiotherapy Department, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, P.R. China.
Emergency Department, Weifang Traditional Chinese Hospital, Weifang, Shandong 261000, P.R. China.
Oncol Lett. 2019 Sep;18(3):3097-3103. doi: 10.3892/ol.2019.10645. Epub 2019 Jul 22.
Gastric cancer (GC), the second most common malignant cancer worldwide, gives rise to a number of cancer-associated fatalities annually. Accumulating evidence has shown that microRNAs (miRs) may serve as oncogenes or tumor suppressors in GC development. The aim of this study was to discover the expression, function and mechanism of miR-761 in GC progression. First, the findings revealed that the expression level of miR-761 was significantly decreased in GC cell lines and tissues. The functional studies showed that miR-761 in GC cells inhibited tumor proliferation and metastasis. In the mechanistic study, through an online database search and luciferase assay, Ras and Rab interactor 1 (RIN1), which has been demonstrated as an oncogene in various types of cancer, including GC, was identified as a target of miR-761. Notably, miR-761 expression was demonstrated to be negatively correlated with RIN1 mRNA levels in GC tissues. Simultaneously, overexpression of RIN1 partially rescued the inhibitory effect of miR-761 mimic in the GC cells. The present study provided new insights into the role of miR-761 in the progression of GC, and implicated the potential application of miR-761 in GC cell therapy.
胃癌（GC）是全球第二常见的恶性肿瘤，每年导致大量癌症相关死亡。越来越多的证据表明，微小RNA（miR）在胃癌发生发展过程中可能作为癌基因或肿瘤抑制因子发挥作用。本研究旨在探究miR-761在胃癌进展中的表达、功能及机制。首先，研究结果显示miR-761在胃癌细胞系和组织中的表达水平显著降低。功能研究表明，胃癌细胞中的miR-761抑制肿瘤增殖和转移。在机制研究中，通过在线数据库搜索和荧光素酶报告基因检测，发现Ras和Rab相互作用分子1（RIN1）是miR-761的靶标，RIN1在包括胃癌在内的多种癌症中已被证明是一种癌基因。值得注意的是，在胃癌组织中，miR-761表达与RIN1 mRNA水平呈负相关。同时，RIN1的过表达部分挽救了miR-761模拟物对胃癌细胞的抑制作用。本研究为miR-761在胃癌进展中的作用提供了新见解，并提示miR-761在胃癌细胞治疗中的潜在应用价值。
