使用落射照明穆勒矩阵显微镜通过微弹性成像测量对生物组织进行定量鉴别。
Quantitative discrimination of biological tissues by micro-elastographic measurement using an epi-illumination Mueller matrix microscope.
作者信息
Mukherjee Pradipta, Horiguchi Tomohisa, Shibata Shuhei, Hagen Nathan, Otani Yukitoshi
机构信息
Graduate School of Engineering, Utsunomiya University, Utsunomiya, Tochigi, 321-8585, Japan.
Center for Optical Research and Education, Utsunomiya University, Utsunomiya, Tochigi, 321-8585, Japan.
出版信息
Biomed Opt Express. 2019 Jul 9;10(8):3847-3859. doi: 10.1364/BOE.10.003847. eCollection 2019 Aug 1.
Abstract
We propose a method for estimating the stiffness of bio-specimens by measuring their linear retardance properties under applied stress. For this purpose, we employ an epi-illumination Mueller matrix microscope and show the procedures for its calibration. We provide experimental results demonstrating how to apply Mueller matrix data to elastography, using chicken liver and chicken heart as biological samples. Finally, we show how the histograms of linear retardance images can be used to distinguish between specimens and quantify the discrimination accuracy.
摘要
我们提出了一种通过测量生物样本在施加应力下的线性延迟特性来估计其刚度的方法。为此，我们使用落射照明穆勒矩阵显微镜，并展示了其校准程序。我们提供了实验结果，展示了如何将穆勒矩阵数据应用于弹性成像，使用鸡肝和鸡心作为生物样本。最后，我们展示了线性延迟图像的直方图如何用于区分样本并量化辨别准确性。
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