Faculty of Agro-Industry, Department of Food Technology, Prince of Songkla University, Hat Yai, Thailand.
J Food Biochem. 2019 Nov;43(11):e13024. doi: 10.1111/jfbc.13024. Epub 2019 Aug 27.
Coconut milk was hydrolyzed by partially purified protease from seabass pyloric caeca (PPSP) and commercial trypsin (CT) at various levels (5 and 10 unit/g protein) at 60°C for different hydrolysis times (0-150 min). At the same protease level and hydrolysis time, higher degrees of hydrolysis and larger droplet size were found in coconut milk hydrolyzed by PPSP, compared to CT. The highest virgin coconut oil (VCO) yield (77.34%) was observed after the sample was hydrolyzed for 150 min by PPSP (10 units/g protein). Protein patterns indicated that coconut milk proteins were more prone to hydrolysis by PPSP, compared to CT. A marked difference was not found in physicochemical properties of commercial VCO and VCO separated from coconut milk using PPSP. Therefore, VCO could be extracted using PPSP under optimal condition, wherein the extraction could be accomplished within a short time with high yield. PRACTICAL APPLICATIONS: In virgin coconut oil (VCO) manufacture, the yield of VCO and cost of the commercial enzyme are of main concern. Therefore, cheap source of proteases, particularly proteases from seabass pyloric caeca, can be a promising alternative for the manufacture of VCO. Therefore, the cost associated with commercial enzymes could be reduced and the proteases from seabass processing byproducts were better exploited.
椰奶经鲈鱼幽门盲囊（PPSP）部分纯化的蛋白酶和商业胰蛋白酶（CT）在不同水平（5 和 10 单位/克蛋白）60°C 下进行水解，水解时间不同（0-150 分钟）。在相同的蛋白酶水平和水解时间下，与 CT 相比，PPSP 水解的椰奶具有更高的水解度和更大的液滴尺寸。在 PPSP（10 单位/克蛋白）水解 150 分钟后，发现初榨椰子油（VCO）的产率最高（77.34%）。蛋白图谱表明，与 CT 相比，椰奶蛋白更易被 PPSP 水解。使用 PPSP 从椰奶中分离出的商业 VCO 和 VCO 的理化性质没有明显差异。因此，VCO 可以在最佳条件下使用 PPSP 提取，在短时间内以高产率完成提取。实际应用：在初榨椰子油（VCO）生产中，VCO 的产量和商业酶的成本是主要关注点。因此，廉价的蛋白酶来源，特别是来自鲈鱼幽门盲囊的蛋白酶，可以作为生产 VCO 的一种有前途的替代方法。因此，可以降低与商业酶相关的成本，并更好地利用来自鲈鱼加工副产物的蛋白酶。
