Department of Applied Chemistry, Institute of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, China.
School of Materials Science and Engineering, South China University of Technology, Guangzhou, China.
Biopolymers. 2019 Dec;110(12):e23328. doi: 10.1002/bip.23328. Epub 2019 Aug 27.
Blood vessels play an important role in bone defect repair and growth, and a critical challenge of bone defect repair is the promotion of blood vessel formation. Most of the current methods promote vascularization by adding specific growth factors, which are costly and easy to inactivate. In this study, we developed a covalently cross-linked aminated bioactive glass nanoparticle-chondroitin sulfate methacrylate (ABGN-CSMA) organic-inorganic composite hydrogel with angiogenic properties. The amino groups of the ABGNs form covalent bonds with the carboxyl groups on CSMA. Surface amination modification of BGNs not only improved the dispersion of BGNs in CSMA but also significantly improved the mechanical properties of the composite hydrogel. The largest storage modulus (1200 Pa), the largest loss modulus (560 Pa) and the strongest resistance to deformation of the hydrogel are seen at 10% concentration of ABGNs. Simultaneously, the local pH stability and sustained ion release of the composite hydrogel are conducive to cell adhesion, proliferation, and angiogenesis. This work provides evidence for the development of covalently cross-linked organic-inorganic composite hydrogels with angiogenic properties.
血管在骨缺损修复和生长中起着重要作用，而骨缺损修复的一个关键挑战是促进血管形成。目前大多数方法通过添加特定的生长因子来促进血管生成，但是这些方法成本高，容易失活。在这项研究中，我们开发了一种具有血管生成特性的共价交联氨基化生物活性玻璃纳米粒子-软骨素硫酸甲基丙烯酰胺（ABGN-CSMA）有机-无机复合水凝胶。ABGN 的氨基与 CSMA 上的羧基形成共价键。BGN 的表面氨基化修饰不仅改善了 BGN 在 CSMA 中的分散性，而且显著提高了复合水凝胶的力学性能。在 ABGN 浓度为 10%时，水凝胶具有最大的储能模量（1200 Pa）、最大的损耗模量（560 Pa）和最强的抗变形能力。同时，复合水凝胶的局部 pH 稳定性和持续的离子释放有利于细胞黏附、增殖和血管生成。这项工作为开发具有血管生成特性的共价交联有机-无机复合水凝胶提供了证据。
