Murmansk Marine Biological Institute, Vladimirskaya St. 17, 183010, Murmansk, Russia; Murmansk Arctic State University, Kapitan Egorov Str. 15, 183038, Murmansk, Russia.
Murmansk Marine Biological Institute, Vladimirskaya St. 17, 183010, Murmansk, Russia.
Environ Pollut. 2019 Nov;254(Pt B):113072. doi: 10.1016/j.envpol.2019.113072. Epub 2019 Aug 17.
The viability and physiological state of brown macroalgae Fucus vesiculosus and its associated epiphytic bacteria exposed to diesel water-accommodated fraction (WAF), as well as the capacity of this association to deplete petroleum hydrocarbons (HCs) were experimentally tested. After a 6-day exposure treatment, the algal-surface associated bacteria were identified as primarily hydrocarbon-oxidising bacteria (HOB), and the algal-HOB association was able to deplete petroleum hydrocarbons from the diesel WAF by 80%. The HOB density on the algal surface exposed to diesel WAF was 350% higher compared to the control (i.e. HOB density on the algal surface exposed to ambient seawater), which suggest that they actively proliferated in the presence of hydrocarbons and most likely consumed hydrocarbons as their primary organic substrate. Exposure to diesel WAF did not affect the metabolic activity of F. vesiculosus. Higher lipid peroxidation was observed in F. vesiculosus exposed to diesel WAF while catalase concentration decreased only during the first day of exposure. Results suggest F. vesiculosus is tolerant to oil pollution and the algal-HOB association can efficiently deplete petroleum hydrocarbons in oil-contaminated seas.
实验测试了暴露在柴油水容纳部分（WAF）中的褐色大型海藻泡叶藻及其相关附生细菌的生存能力和生理状态，以及这种共生体消耗石油烃（HCs）的能力。经过 6 天的暴露处理，鉴定出藻类表面相关细菌主要为烃氧化菌（HOB），藻类-HOB 共生体能够从柴油 WAF 中消耗 80%的石油烃。与对照（即暴露于环境海水中的藻类表面上的 HOB 密度）相比，暴露于柴油 WAF 的藻类表面上的 HOB 密度高 350%，这表明它们在烃存在的情况下积极增殖，并且很可能将烃作为其主要有机底物消耗。暴露于柴油 WAF 并未影响泡叶藻的代谢活性。在暴露于柴油 WAF 的泡叶藻中观察到更高的脂质过氧化，而过氧化氢酶浓度仅在暴露的第一天就下降。结果表明，泡叶藻对石油污染具有耐受性，藻类-HOB 共生体可以有效地消耗受石油污染海域中的石油烃。
