Mazón-Suástegui José Manuel, Salas-Leiva Joan, Teles Andressa, Tovar-Ramírez Dariel
Centro de Investigaciones Biológicas del Noroeste S.C. (CIBNOR), La Paz, Baja California Sur, Mexico.
Homeopathy. 2020 Feb;109(1):3-13. doi: 10.1055/s-0039-1692998. Epub 2019 Aug 27.
This research aimed to observe the effect of homeopathically prepared (ViP) and (ViA) and the commercial homeopathic compound Similia ( and ) on the digestive enzyme activities of juveniles under usual culture conditions.
Biochemical analysis was used to study the effect of highly diluted substances (7C potency) prepared from ViP and ViA (Treatment 1: T1) and the homeopathic compound and (Treatment 2: T2) on changes in the main digestive enzymes on weaning-state fish (WS; 30 days post-hatching [DPH]) and early juveniles (EJ; 62 DPH) a reference control group that received no homeopathic medicines.
Treatment T2 significantly increased the activity of trypsin and lipase and decreased the activity of amylase, whereas treatment T1 increased the activity of chymotrypsin and reduced the activity of aminopeptidase-N in WS fish. Except for alkaline phosphatase, which was significantly reduced in the intestine, no significant differences in enzymatic activity were found between treated EJ fish and controls. The fish of the WS group had a higher growth rate with the T2 treatment.
T1 treatment stimulated chymotrypsin in EJ fish and T2 promoted intestinal maturation of WS fish. Higher growth rate with the T2 treatment may be associated with the stimulation of trypsin activity. Thus, T2 may be applied, under hatchery conditions, during larval stages with an aim to enhance digestion and assimilation of inert food.
本研究旨在观察顺势制备的（ViP）和（ViA）以及商业顺势复方Similia（和）在常规养殖条件下对幼鱼消化酶活性的影响。
采用生化分析方法，研究从ViP和ViA制备的高稀释物质（7C效力）（处理1：T1）以及顺势复方和（处理2：T2）对断奶期鱼（WS；孵化后30天[DPH]）和早期幼鱼（EJ；62 DPH）主要消化酶变化的影响，设置一个未接受顺势药物的参考对照组。
处理T2显著提高了胰蛋白酶和脂肪酶的活性，降低了淀粉酶的活性，而处理T1提高了糜蛋白酶的活性，并降低了WS鱼中氨肽酶-N的活性。除肠道中碱性磷酸酶显著降低外，处理后的EJ鱼与对照组之间的酶活性未发现显著差异。WS组的鱼在T2处理下生长速度更快。
T1处理刺激了EJ鱼中的糜蛋白酶，T2促进了WS鱼的肠道成熟。T2处理下更高的生长速度可能与胰蛋白酶活性的刺激有关。因此，在孵化场条件下，T2可在幼体阶段应用，以增强对惰性食物消化和吸收。
