Veyrand-Quirós Bernardo, Guzmán-Villanueva Laura T, Reyes Ana G, Rodríguez-Jaramillo Carmen, Salas-Leiva Joan S, Tovar-Ramírez Dariel, Balcázar José L, Quiroz-Guzman Eduardo
Centro de Investigaciones Biológicas del Noroeste S.C., Av. Instituto Politécnico Nacional 195. Col. Playa Palo de Santa Rita Sur, 23096. La Paz, Baja California Sur, Mexico.
CONACYT-CIBNOR, Av. Instituto Politécnico Nacional 195. Col. Playa Palo de Santa Rita Sur, 23096. La Paz, Baja California Sur, Mexico.
Appl Microbiol Biotechnol. 2021 Apr;105(7):2877-2887. doi: 10.1007/s00253-021-11223-z. Epub 2021 Mar 12.
The Seriola genus includes species of worldwide commercial importance due to its rapid growth and easy adaptability to confinement conditions. However, like other fish species, large mortalities occur during their early life stages, where the main problems are caused by opportunistic bacteria. Disease control strategies are thus urgently needed. The present study aimed to evaluate the efficacy of phage vB_Pd_PDCC-1 during the early development of longfin yellowtail (Seriola rivoliana), as well as its effect on microbial communities. This broad-host-range phage was added to the culture every 3 days starting from the egg-stage until 12 days after hatching (DAH) at a concentration of 1.41×10 plaque-forming units (PFU) per mL and at a multiplicity of infection (MOI) of 1. The results showed positive effects (p<0.05) on egg hatching, survival, growth, and pigmentation area in treated larvae. Moreover, high-throughput sequencing analysis of 16S rRNA genes showed that phage administration did not produce significant changes (p>0.05) in the composition and structure of the associated microbiota. However, sequences affiliated to the Gammaproteobacteria class were displaced by those belonging to the Alphaproteobacteria class over time regardless of the treatment received. At the family level, there was a decrease in Rhodobacteraceae, Pseudoalteromonadaceae, and Flavobacteriaceae in both groups over time. To our best knowledge, this study represents the first attempt to evaluate the effect of a phage as a biological control agent during ontogenetic development of longfin yellowtail larvae. KEY POINTS: • Phages can be used against proliferation of Vibrio in fish cultures. • Seriola includes several important commercial fish species due to its rapid growth. • Phages do not cause significant changes in the associated microbiota.
由于其生长迅速且易于适应圈养条件，黄尾鰤属的鱼类具有全球商业重要性。然而，与其他鱼类一样，它们在幼鱼阶段会出现大量死亡，主要问题是由机会性细菌引起的。因此，迫切需要疾病控制策略。本研究旨在评估噬菌体vB_Pd_PDCC-1在长鳍黄尾鰤（Seriola rivoliana）早期发育过程中的功效及其对微生物群落的影响。从卵期开始，每隔3天向养殖环境中添加这种广宿主范围的噬菌体，直至孵化后12天（DAH），浓度为每毫升1.41×10噬菌斑形成单位（PFU），感染复数（MOI）为1。结果显示，处理后的幼虫在卵孵化、存活、生长和色素沉着面积方面有积极影响（p<0.05）。此外，16S rRNA基因的高通量测序分析表明，施用噬菌体对相关微生物群的组成和结构没有产生显著变化（p>0.05）。然而，无论接受何种处理，随着时间的推移，与γ-变形菌纲相关的序列都被属于α-变形菌纲的序列所取代。在科水平上，随着时间的推移，两组中的红杆菌科、假交替单胞菌科和黄杆菌科都有所减少。据我们所知，本研究是首次尝试评估噬菌体作为生物控制剂在长鳍黄尾鰤幼体个体发育过程中的作用。关键点：• 噬菌体可用于对抗鱼类养殖中弧菌的增殖。• 由于生长迅速，黄尾鰤属包括几种重要的商业鱼类。• 噬菌体不会引起相关微生物群的显著变化。
