Ghoreyshi Seyed Mohammad, Omri Besma, Chalghoumi Raja, Bouyeh Mehrdad, Seidavi Alireza, Dadashbeiki Mohammad, Lucarini Massimo, Durazzo Alessandra, Hoven Rene van den, Santini Antonello
Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht 43, Iran.
Laboratory of Improvement and Integrated Development of Animal Productivity and Food Resources, Department of Animal Science, College of Agriculture of Mateur, University of Carthage, Bizerte 7000, Tunisia.
Animals (Basel). 2019 Aug 26;9(9):608. doi: 10.3390/ani9090608.
L-carnitine as well as lysine and methionine are amino acids of important nutritional and nutraceutical interest and are used in nutritional strategies as dietary supplements to improve feed quality characteristics in animals and broiler chicken in particular. This study investigated the effect of different levels of L-carnitine and extra levels of lysine-methionine on growth performance, carcass characteristics, and some immune system markers. Two hundred seventy male Ross 308 broilers were a fed control diet (C) and eight different diets supplemented with an excess of amino acids. In the experimental diets, identified as D1, D2, D3, D4, D5, D6, D7, and D8, extra L-carnitine, lysine, and methionine were added in excess with respect to the American National Research Council (NRC) recommendations: L-carnitine equal to NRC (D1), control diet supplemented with lysine at 30% in excess of NRC, methionine at 30% in excess of NRC, and L-carnitine equal to NRC (D2), control diet supplemented with lysine equal to NRC, methionine equal to NRC, and L-carnitine at 15% in excess of NRC (D3), control diet supplemented control diet supplemented with lysine at 15% in excess of NRC, methionine at 15% in excess of NRC, and L-carnitine at 15% in excess of NRC (D4), control diet supplemented lysine at 30% in excess of NRC, methionine at 30% in excess of NRC, and L-carnitine at 15% in excess of NRC (D5), control diet supplemented with lysine equal to NRC recommendations, methionine equal to NRC recommendations, and L-carnitine at 75% in excess of NRC (D6), control diet supplemented with lysine at 15% in excess of NRC, methionine at 15% in excess of NRC, and L-carnitine at 75% in excess of NRC (D7); and control diet supplemented with lysine at 30% in excess of NRC, methionine at 30% in excess of NRC, and L-carnitine at 75% in excess of NRC (D8). During the starter and growth phases, feed intake was not affected by dietary treatment ( > 0.05). By contrast, body weight and FCR were both affected ( < 0.01) during the starter period. During the finisher phase, feed consumption was affected ( < 0.05) by dietary treatment. Feed intake of broilers fed on C, D3, D6, and D7 were statistically similar ( > 0.05) (1851.90, 1862.00, 1945.10, and 1872.80 g/pen/day, respectively) and were higher ( < 0.05) than 1564.40 g/pen/day (D5). With the exception of drumsticks, neck, back thoracic vertebrae, and proventriculus weights, the economical carcass segments were not affected ( > 0.05) by the dietary supplementation of amino acids. Duodenum and ileum weights and lengths decreased with amino acid supplementation ( < 0.05). IgT and IgG titers against Sheep Red Blood Cells (SRBC) for both primary and secondary responses were not affected by dietary treatments ( > 0.05). Dietary amino acids supplementation did not affect IgM titer after the secondary challenge ( > 0.05) and had a significant effect ( < 0.05) on serum antibody titers in broilers vaccinated against Newcastle disease (NCD) and Gumboro 's disease at the 27th and 30th days, respectively.
左旋肉碱以及赖氨酸和蛋氨酸都是具有重要营养和营养保健意义的氨基酸,在营养策略中用作膳食补充剂,以改善动物尤其是肉鸡的饲料质量特性。本研究调查了不同水平的左旋肉碱以及额外添加的赖氨酸 - 蛋氨酸对生长性能、胴体特性和一些免疫系统标志物的影响。270只雄性罗斯308肉鸡被喂食对照日粮(C)和八种添加了过量氨基酸的不同日粮。在实验日粮中,分别标记为D1、D2、D3、D4、D5、D6、D7和D8,相对于美国国家研究委员会(NRC)的建议,额外添加了左旋肉碱、赖氨酸和蛋氨酸:左旋肉碱含量等于NRC(D1),对照日粮添加的赖氨酸比NRC过量30%,蛋氨酸比NRC过量30%,左旋肉碱含量等于NRC(D2),对照日粮添加的赖氨酸等于NRC,蛋氨酸等于NRC,左旋肉碱比NRC过量15%(D3),对照日粮添加的赖氨酸比NRC过量15%,蛋氨酸比NRC过量15%,左旋肉碱比NRC过量15%(D4),对照日粮添加的赖氨酸比NRC过量30%,蛋氨酸比NRC过量30%,左旋肉碱比NRC过量15%(D5),对照日粮添加的赖氨酸等于NRC建议量,蛋氨酸等于NRC建议量,左旋肉碱比NRC过量75%(D6),对照日粮添加的赖氨酸比NRC过量15%,蛋氨酸比NRC过量15%,左旋肉碱比NRC过量75%(D7);对照日粮添加的赖氨酸比NRC过量30%,蛋氨酸比NRC过量30%,左旋肉碱比NRC过量75%(D8)。在育雏期和生长期,采食量不受日粮处理的影响(P>0.05)。相比之下,在育雏期体重和饲料转化率均受到影响(P<0.01)。在育肥期,采食量受日粮处理的影响(P<0.05)。喂食C、D3、D6和D7日粮的肉鸡采食量在统计学上相似(P>0.05)(分别为1851.90、1862.00、1945.10和1872.80克/栏/天),且高于1564.40克/栏/天(D5)(P<0.05)。除鸡腿、颈部、胸段脊椎和腺胃重量外,经济胴体部分不受氨基酸日粮添加的影响(P>0.05)。十二指肠和回肠的重量和长度随着氨基酸添加而减少(P<0.05)。针对绵羊红细胞(SRBC)的初次和二次应答的IgT和IgG滴度不受日粮处理的影响(P>0.05)。日粮添加氨基酸对二次攻击后的IgM滴度没有影响(P>0.05),并且分别在第27天和第30天对接种新城疫(NCD)和甘保罗病疫苗的肉鸡血清抗体滴度有显著影响(P<0.05)。