Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Center of Systems Biology and Human Health, School of Science and Institute for Advanced Study, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Development. 2019 Sep 20;146(18):dev179754. doi: 10.1242/dev.179754.
Organ formation relies on the orchestration of pattern formation, proliferation and growth during development. How these processes are integrated at the individual cell level remains unclear. In the past decades, studies using wing imaginal discs as a model system have provided valuable insights into pattern formation, growth control and regeneration. Here, we provide single cell transcriptomic landscapes of pattern formation, proliferation and growth of wing imaginal discs. We found that patterning information is robustly maintained in the single cell transcriptomic data and can provide reference matrices for computationally mapping single cells into discrete spatial domains. Assignment of wing disc single cells to spatial subregions facilitates examination of patterning refinement processes. We also clustered single cells into different proliferation and growth states and evaluated the correlation between cell proliferation/growth states and spatial patterning. Furthermore, single cell transcriptomic analyses allowed us to quantitatively examine disturbances of differentiation, proliferation and growth in a well-established tumor model. We provide a database to explore these datasets at http://drosophilayanlab-virtual-wingdisc.ust.hk:3838/v2/This article has an associated 'The people behind the papers' interview.
器官的形成依赖于发育过程中模式形成、增殖和生长的协调。这些过程如何在单个细胞水平上整合仍不清楚。在过去的几十年中，使用 翅 成虫盘作为模型系统的研究为模式形成、生长控制和再生提供了有价值的见解。在这里，我们提供了翅成虫盘模式形成、增殖和生长的单细胞转录组图谱。我们发现，在单细胞转录组数据中，模式信息得到了稳健的维持，并可以为计算将单细胞映射到离散空间域提供参考矩阵。将翅盘单细胞分配到空间亚区有助于检查模式细化过程。我们还将单细胞聚类为不同的增殖和生长状态，并评估细胞增殖/生长状态与空间模式之间的相关性。此外，单细胞转录组分析使我们能够定量检查在一个成熟的肿瘤模型中分化、增殖和生长的干扰。我们提供了一个数据库，可在 http://drosophilayanlab-virtual-wingdisc.ust.hk:3838/v2/ 上探索这些数据集。本文有一个相关的“论文背后的人物”访谈。
