Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 201602, China.
University of the Chinese Academy of Sciences, Beijing, 100049, China.
Plant Cell Rep. 2019 Dec;38(12):1465-1471. doi: 10.1007/s00299-019-02457-3. Epub 2019 Aug 27.
Agrobacterium-mediated transient overexpression of E2Fb triggers new cell divisions in pavement cells of Nicotiana benthamiana leaves. Transient transformation in Nicotiana benthamiana enables the study of multiple biological processes in a simple and fast manner. Here, we describe that, upon A. tumefaciens-mediated transient overexpression of the cell cycle regulator E2Fb from either Arabidopsis thaliana or N. benthamiana, cell divisions occur in epidermal pavement cells in N. benthamiana leaves, following a sequence of events that encompasses the nucleus taking a central position and being surrounded by chloroplasts, nuclear division, and formation of a new wall that divides the initial cell in two. Our results indicate that transient expression in N. benthamiana can be used to study cell division in plants, from DNA replication to cell wall formation, in a simple, controlled, and rapid manner.
农杆菌介导的 E2Fb 瞬时过表达触发拟南芥叶表皮细胞的新细胞分裂。在本氏烟中的瞬时转化可以以简单、快速的方式研究多个生物学过程。在这里，我们描述了，在农杆菌介导的拟南芥或本氏烟细胞周期调控因子 E2Fb 的瞬时过表达后，细胞分裂发生在本氏烟叶片的表皮细胞中，遵循一系列事件，包括细胞核占据中心位置并被叶绿体包围、核分裂和形成新的细胞壁，将初始细胞一分为二。我们的结果表明，在本氏烟中的瞬时表达可以用于以简单、可控和快速的方式研究植物中的细胞分裂，从 DNA 复制到细胞壁形成。
