Biochemistry, Royal Holloway University of London, Egham, UK.
Transgenic Res. 2021 Jun;30(3):303-315. doi: 10.1007/s11248-021-00256-9. Epub 2021 Apr 28.
Over the recent years, Nicotiana benthamiana has gained great importance as a chassis for the production of high value, low volume pharmaceuticals and/or active pharmaceutical ingredients (APIs). The process involving infiltration of the N. benthamiana leaves with Agrobacterium spp, harbouring vectors with the gene of interest, facilitates transient expression. To date, little information is available on the effect of the agro-infiltration process on the metabolome of N. benthamiana, which is necessary to improve the process for large-scale, renewable manufacturing of high value compounds and medical products. Hence, the objective of the present study was to assess metabolic adaptation of N. benthamiana as a response to the presence of Agrobacterium. The present study elucidated changes of the steady-state metabolism in the agroinfiltrated leaf area, the area around the infection and the rest of the plant. Furthermore, the study discusses the phenotypic advantages of the N. benthamiana lab strain, optimised for agro-infiltration, compared to three other wild accessions. Results showed that the lab strain has a different metabolic composition and showed less alterations of the phenylpropanoid pathway and cell wall remodelling in the agroinfiltrated leaf areas, for example chlorogenic acid, cadaverine and C18:0-2-glycerol ester. In conclusion, both of these alterations present potential candidates to improve the phenotype of the N. benthamiana lab strain for a more efficient transient expression process.
近年来，Nicotiana benthamiana 作为生产高价值、低体积药物和/或活性药物成分 (API) 的底盘得到了极大的重视。通过用携带感兴趣基因的农杆菌属（Agrobacterium spp）浸润 N. benthamiana 叶片的过程，促进了瞬时表达。迄今为止，关于 agro-infiltration 过程对 N. benthamiana 代谢组的影响的信息很少，这对于改进大规模、可再生的高价值化合物和医疗产品制造过程是必要的。因此，本研究的目的是评估 N. benthamiana 的代谢适应作为对农杆菌属存在的反应。本研究阐明了 agro-infiltrated 叶片区域、感染区域周围和植物其余部分的稳态代谢变化。此外，该研究还讨论了优化用于 agro-infiltration 的 N. benthamiana 实验室菌株与其他三个野生品系相比的表型优势。结果表明，实验室菌株具有不同的代谢组成，在 agro-infiltrated 叶片区域，例如绿原酸、尸胺和 C18:0-2-甘油酯，苯丙素途径和细胞壁重塑的变化较小。总之，这些变化都为改善 N. benthamiana 实验室菌株的表型提供了潜在的候选物，以提高瞬时表达过程的效率。
