Department of Otolaryngology, Renmin Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, People's Republic of China.
Department of Otolaryngology Head and Neck Surgery, The First People's Hospital of Yinchuan, Yinchuan, China.
Eur Arch Otorhinolaryngol. 2019 Nov;276(11):3131-3138. doi: 10.1007/s00405-019-05608-0. Epub 2019 Aug 27.
We investigated the correlation between the expression of IL-17A in nasopharyngeal carcinoma tissues and cells and the occurrence and development of NPC was also investigated.
Forty-five NPC biopsy specimens from January 2014 to January 2016 were selected. Forty-five NPC tissue specimens and 45 chronic nasopharyngitis tissue samples were detected by immunohistochemistry. Statistical methods were used to analyze the correlation between IL-17A expression and the clinicopathological variables of NPC. The NPC patients were followed up. The levels of IL-17A mRNA in 40 NPC tissue specimens and 45 chronic nasopharyngitis tissue samples were detected by real-time PCR. IL-17A expression in 15 NPC tissue specimens and chronic nasopharyngitis tissue samples was further detected by Western blotting assays.
IL-17A expression in NPC tissues was significantly higher than that of chronic nasopharyngitis tissues (P < 0.05). IL-17A was expressed in the nucleus and cytoplasm of both NPC tissues and chronic nasopharyngitis tissues. Stage III + IV NPC, tumor volume ≥ 50 mm, and hepatic envelope invasion and cervical lymph node metastasis were associated with significantly higher IL-17A levels versus stage I + II NPC, tumor size < 50 mm, no membrane invasion and lack of cervical lymph node metastasis (P < 0.05). IL-17A was statistically associated with tissue differentiation, serum EBV-lgA levels, and EBV infection. IL-17A-positive patients had significantly longer median survival versus IL-17A-negative patients (21.0 vs. 13.0 months, log-rank test: P < 0.05). Furthermore, 65% (26/40) of NPC tissue samples had significantly higher IL-17A mRNA levels than chronic nasopharyngitis (P < 0.05). IL-17A expression was significantly higher in NPC ≥ 50 mm, stage III + IV NPC and NPC with cervical lymph node invasion than its corresponding chronic nasopharyngitis tissue.
IL-17A may be involved in the regulation of various malignant biological behaviors of NPC, which is closely related to the occurrence and development of NPC.
研究白细胞介素-17A（IL-17A）在鼻咽癌组织和细胞中的表达与 NPC 的发生发展的相关性。
选取 2014 年 1 月至 2016 年 1 月期间 45 例鼻咽癌活检标本，采用免疫组织化学法检测 45 例鼻咽癌组织标本和 45 例慢性鼻咽炎组织标本，统计分析 IL-17A 表达与 NPC 临床病理变量的相关性。对 40 例 NPC 组织标本和 45 例慢性鼻咽炎组织标本进行实时荧光定量 PCR 检测 IL-17A mRNA 水平，进一步采用 Western blot 检测 15 例 NPC 组织标本和慢性鼻咽炎组织标本中 IL-17A 的表达。
NPC 组织中 IL-17A 的表达明显高于慢性鼻咽炎组织（P<0.05）。IL-17A 在 NPC 组织和慢性鼻咽炎组织的细胞核和细胞质中均有表达。Ⅲ+Ⅳ期 NPC、肿瘤体积≥50mm、包膜侵犯和颈淋巴结转移患者的 IL-17A 水平明显高于Ⅰ+Ⅱ期 NPC、肿瘤体积<50mm、无包膜侵犯和无颈淋巴结转移患者（P<0.05）。IL-17A 与组织分化、血清 EBV-lgA 水平和 EBV 感染有关。IL-17A 阳性患者的中位生存期明显长于 IL-17A 阴性患者（21.0 个月比 13.0 个月，log-rank 检验：P<0.05）。此外，40 例 NPC 组织标本中有 65%（26/40）的 IL-17A mRNA 水平明显高于慢性鼻咽炎（P<0.05）。在肿瘤≥50mm、Ⅲ+Ⅳ期 NPC 和有颈淋巴结侵犯的 NPC 中，IL-17A 的表达明显高于相应的慢性鼻咽炎组织。
IL-17A 可能参与调节 NPC 的多种恶性生物学行为，与 NPC 的发生发展密切相关。
