Mechanical Engineering, University of Michigan, 1049 Bonisteel Interdisciplinary Research Building, 2360 Bonisteel Boulevard, Ann Arbor, MI, 48109-2099, USA.
Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Ann Biomed Eng. 2020 Jan;48(1):329-341. doi: 10.1007/s10439-019-02350-w. Epub 2019 Aug 27.
Although widely used as a preclinical model for studying cardiovascular diseases, there is a scarcity of in vivo hemodynamic measurements of the naïve murine system in multiple arterial and venous locations, from head-to-toe, and across sex and age. The purpose of this study is to quantify cardiovascular hemodynamics in mice at different locations along the vascular tree while evaluating the effects of sex and age. Male and female, adult and aged mice were anesthetized and underwent magnetic resonance imaging. Data were acquired from four co-localized vessel pairs (carotid/jugular, suprarenal and infrarenal aorta/inferior vena cava (IVC), femoral artery/vein) at normothermia (core temperature 37 ± 0.2 °C). Influences of age and sex on average velocity differ by location in arteries. Average arterial velocities, when plotted as a function of distance from the heart, decrease nearly linearly from the suprarenal aorta to the femoral artery (adult and aged males: - 0.33 ± 0.13, R= 0.87; - 0.43 ± 0.10, R = 0.95; adult and aged females: - 0.23 ± 0.07, R = 0.91; - 0.23 ± 0.02, R = 0.99). Average velocity of aged males and average volumetric flow of aged males and females tended to be larger compared to adult comparators. With cardiovascular disease as the leading cause of death and with the implications of cardiovascular hemodynamics as important biomarkers for health and disease, this work provides a foundation for sex and age comparisons in pathophysiology by collecting and analyzing hemodynamic data for the healthy murine arterial and venous system from head-to-toe, across sex and age.
尽管在研究心血管疾病方面被广泛用作临床前模型，但在从头至脚的多个动脉和静脉位置以及跨性别和年龄的情况下，对原始鼠系统的体内血液动力学测量却很少。本研究的目的是在评估性别和年龄影响的同时，定量测量血管树中不同位置的心血管血液动力学。雄性和雌性成年和老年小鼠麻醉后接受磁共振成像。在正常体温（核心温度 37±0.2°C）下，从四个共定位血管对（颈动脉/颈静脉，肾动脉上方和下方的主动脉/下腔静脉（IVC），股动脉/静脉）采集数据。年龄和性别的平均速度的影响因动脉的位置而异。当将平均动脉速度绘制为距心脏的距离的函数时，从肾动脉上方到股动脉的速度几乎呈线性下降（成年和老年雄性：-0.33±0.13，R=0.87；-0.43±0.10，R=0.95；成年和老年雌性：-0.23±0.07，R=0.91；-0.23±0.02，R=0.99）。与成年对照相比，老年雄性的平均速度和老年雄性和雌性的平均容积流量趋于更大。由于心血管疾病是导致死亡的主要原因，并且心血管血液动力学作为健康和疾病的重要生物标志物，因此本工作通过从头至脚收集和分析健康鼠动静脉系统的血液动力学数据，为跨性别和年龄的病理生理学比较提供了基础。
