Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, Gansu, China.
Department of Integrated Chinese and Western Medicine Gynecology, Gansu Provincial Maternity and Child-care Hospital, Lanzhou, China.
Environ Toxicol. 2020 Jan;35(1):37-46. doi: 10.1002/tox.22840. Epub 2019 Aug 27.
Phenanthrene (Phe) female rat model was established to explore the effects of Phe on oxidative stress and inflammation. The rats were randomly divided into three groups including control (C), low (L), and high (H) group. Phe was supplied to L and H groups at the dosage of 180 mg/kg and 900 mg/kg orally at first day, and with the dose 90 mg/kg and 450 mg/kg by intraperitoneal injection at the last 2 days. The C group was enriched with the same volume of corn oil. The blood, lung, and liver tissues were collected. The superoxide dismutase (SOD), malonaldehyde (MDA), and 8-hydroxy-2-deoxyguanosine (8-OHdG) were detected to evaluate oxidative stress. The protein and mRNA expressions of interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), and interleukin 10 (IL-10) were detected to evaluate inflammation. Further, the forkhead box transcription factor 3 (Foxp3) was analyzed to hint the injury mechanism of inflammation. The results showed SOD and MDA in lung and liver, and serum 8-OHdG elevated significantly in H groups (P < .05). Meanwhile, there were significant increases in the protein and mRNA expression of TNF-α and IL-6 in lung and liver of H groups (P < .05). In addition, the protein and mRNA expressions of TGF-β and Foxp3 were all decreased significantly in both lung and liver of H groups (P < .05). Results demonstrated that an obvious change of Phe exposure could induce oxidative stress and inflammation in female rats. This is a first pilot study to explore the association between Phe exposure and oxidative stress and inflammation using a female rat model.
采用雌性大鼠建立菲（Phe）染毒模型，探讨 Phe 对氧化应激和炎症的影响。将大鼠随机分为 3 组，分别为对照组（C 组）、低剂量组（L 组）和高剂量组（H 组）。第 1 天，L 组和 H 组大鼠分别以 180mg/kg 和 900mg/kg 经口灌胃给予 Phe，最后 2 天分别以 90mg/kg 和 450mg/kg 经腹腔注射给予 Phe；C 组给予等体积玉米油。收集血液、肺脏和肝脏组织。检测超氧化物歧化酶（SOD）、丙二醛（MDA）和 8-羟基-2-脱氧鸟苷（8-OHdG）评估氧化应激，检测白细胞介素 6（IL-6）、肿瘤坏死因子-α（TNF-α）、转化生长因子-β（TGF-β）和白细胞介素 10（IL-10）的蛋白和 mRNA 表达评估炎症，进一步分析叉头框转录因子 3（Foxp3）提示炎症损伤机制。结果表明，H 组大鼠肺脏和肝脏 SOD 和 MDA 以及血清 8-OHdG 显著升高（P<.05）。同时，H 组大鼠肺脏和肝脏 TNF-α和 IL-6 的蛋白和 mRNA 表达显著增加（P<.05）。此外，H 组大鼠肺脏和肝脏 TGF-β和 Foxp3 的蛋白和 mRNA 表达均显著降低（P<.05）。结果表明，Phe 暴露量的明显变化可导致雌性大鼠发生氧化应激和炎症。这是首次采用雌性大鼠模型研究 Phe 暴露与氧化应激和炎症之间关系的初步研究。
