Babaskina Liudmila Ivanovna, Litvinova Tatiana Mikhailovna, Babaskin Dmitrii Vladimirovich, Krylova Olga Valerevna
Sechenov First Moscow State Medical University 8-2, Trubetskaya Street, Moscow, Russian Federation.
Open Access Maced J Med Sci. 2019 Jul 14;7(13):2079-2083. doi: 10.3889/oamjms.2019.607. eCollection 2019 Jul 15.
The scientific substantiation for the selection of therapeutically significant dosage of phytocomplex in the dosage form for phonophoresis, control over the delivery of active substances into the body, and what affects this process require the study of the kinetics of phytocomplex flavonoids delivery during phonophoresis.
The aim was to study the possibilities of controlling the process of transdermal delivery of phytocomplex active substances (flavonoids) during phonophoresis in vitro model experiments.
Working compositions with different concentrations of phytocomplex for phonophoresis were used. The content of flavonoids in the compositions was determined using the spectrophotometric method and was calculated equivalent to quercetin, the flavonoid prevailing in the phytocomplex. The study of the kinetics of flavonoids delivery from working compositions was carried out using Franz diffusion cells and Carbosyl-P membranes. The authors determined the main parameters of the process and established the dependence of the delivery rate of flavonoids on their initial concentration in the working composition. The authors studied the effect of dimethyl sulfoxide and the base-forming substances of the working composition on the kinetics of phytocomplex flavonoid delivery during phonophoresis.
The authors recorded an increase in the rate of delivery of the active substances from working compositions containing dimethyl sulfoxide into the model medium by almost 1.5-2 times during the first ten minutes of the experiment (approximate duration of the phonophoresis procedure). The authors proposed technological techniques for improvement of the phonophoresis method for the phytocomplex. The possibilities of control over the process of transdermal delivery of the phytocomplex active ingredients during phonophoresis in vitro model experiments were shown.
The obtained results provide information for further pharmacological studies of the nature and mechanism of the effect of phytocomplex flavonoids during phonophoresis in the rehabilitation of patients with osteoarthrosis.
选择用于离子导入剂型的具有治疗意义的植物复合物剂量、控制活性物质进入体内的过程以及影响该过程的因素，需要研究离子导入过程中植物复合物类黄酮的递送动力学。
目的是在体外模型实验中研究控制离子导入过程中植物复合物活性物质（类黄酮）经皮递送过程的可能性。
使用了不同浓度的用于离子导入的植物复合物工作组合物。采用分光光度法测定组合物中类黄酮的含量，并计算为相当于槲皮素（植物复合物中占主导的类黄酮）。使用Franz扩散池和Carbosyl - P膜研究工作组合物中类黄酮的递送动力学。作者确定了该过程的主要参数，并建立了类黄酮递送速率与其在工作组合物中的初始浓度之间的关系。作者研究了二甲基亚砜和工作组合物的碱形成物质对离子导入过程中植物复合物类黄酮递送动力学的影响。
作者记录到，在实验的前十分钟（离子导入过程的大致持续时间）内，含有二甲基亚砜的工作组合物中活性物质向模型介质中的递送速率增加了近1.5 - 2倍。作者提出了改进植物复合物离子导入方法的技术工艺。展示了在体外模型实验中控制离子导入过程中植物复合物活性成分经皮递送过程的可能性。
所获得的结果为进一步开展关于植物复合物类黄酮在骨关节炎患者康复中的离子导入作用性质和机制的药理学研究提供了信息。
