Gribble Adam, Pinkert Michael A, Westreich Jared, Liu Yuming, Keikhosravi Adib, Khorasani Mohammadali, Nofech-Mozes Sharon, Eliceiri Kevin W, Vitkin Alex
1Department of Medical Biophysics, University of Toronto, Toronto, Canada.
2Laboratory for Optical and Computational Instrumentation, Department of Biomedical Engineering, University of Wisconsin at Madison, Madison, USA.
Biomed Eng Lett. 2019 Jun 20;9(3):339-349. doi: 10.1007/s13534-019-00116-w. eCollection 2019 Aug.
Mueller polarimetry is a quantitative polarized light imaging modality that is capable of label-free visualization of tissue pathology, does not require extensive sample preparation, and is suitable for wide-field tissue analysis. It holds promise for selected applications in biomedicine, but polarimetry systems are often constrained by limited end-user accessibility and/or long-imaging times. In order to address these needs, we designed a multiscale-polarimetry module that easily couples to a commercially available stereo zoom microscope. This paper describes the module design and provides initial polarimetry imaging results from a murine preclinical breast cancer model and human breast cancer samples. The resultant polarimetry module has variable resolution and field of view, is low-cost, and is simple to switch in or out of a commercial microscope. The module can reduce long imaging times by adopting the main imaging approach used in pathology: scanning at low resolution to identify regions of interest, then at high resolution to inspect the regions in detail. Preliminary results show how the system can aid in region of interest identification for pathology, but also highlight that more work is needed to understand how tissue structures of pathological interest appear in Mueller polarimetry images across varying spatial zoom scales.
穆勒偏振测量法是一种定量偏振光成像方式，能够对组织病理学进行无标记可视化，无需进行大量样本制备，适用于宽视野组织分析。它在生物医学的特定应用中具有前景，但偏振测量系统往往受到终端用户可及性有限和/或成像时间长的限制。为了满足这些需求，我们设计了一个多尺度偏振测量模块，它可以轻松地与市售立体变焦显微镜耦合。本文描述了该模块的设计，并提供了来自小鼠临床前乳腺癌模型和人类乳腺癌样本的初始偏振测量成像结果。所得的偏振测量模块具有可变分辨率和视野，成本低，并且可以简单地在商用显微镜中切换接入或移除。该模块可以通过采用病理学中使用的主要成像方法来减少长时间成像：先以低分辨率扫描以识别感兴趣区域，然后以高分辨率检查这些区域的细节。初步结果表明该系统如何有助于病理学中感兴趣区域的识别，但也突出表明，需要开展更多工作来了解在不同空间变焦尺度下，病理学感兴趣的组织结构在穆勒偏振测量图像中是如何呈现的。
