Zain Maryam, Yasmin Sumera, Hafeez Fouzia Yousaf
Department of Biochemistry and Biotechnology, The Women University, Multan, Pakistan.
National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
Iran J Biotechnol. 2019 Apr 20;17(2):e1974. doi: 10.21859/ijb.1974. eCollection 2019 Apr.
Plant Growth Promoting Rhizobacteria (PGPR) may be utilized to augment plant growth and suppress the plant pathogens. Objective: The present study was conducted to isolate and characterize the antagonistic bacteria indigenous to cotton and sugarcane rhizosphere in Pakistan, and to evaluate their ability to suppress phytopathogenic spp. Out of 63 isolates 37 different morphotypes were studied for their antagonistic activity against , and Among these 31 strains showed the percentage suppression ranging from 40 to 66% against spp.
The antagonistic bacteria having antifungal activity were studied for different morphological and physiological characteristics using Gram staining and light microscopy. Most of them were Gram negative and tentatively identified as spp. The selected strains were screened for plant growth regulation and antifungal traits.
Our study included 1000 premature CAD patients that classified into two groups with history of MI (n = 461) and without of MI (n = 539). The polymorphism variants in 10% of samples were determined by PCR-RFLP technique and genotyping of the polymorphism in all subjects was conducted by High Resolution Melting method. Given the two conditions of patients residing in Tehran and also faced with their first episode of MI, 640 out of 1000 study samples that had been previously followed-up were assessed in a retrospective cohort phase regarding long-term major adverse cardiac events (MACE).
Four bacterial strains were able to produce the chitinase enzyme while four other bacterial strains showed protease production. Ten strains were positive for HCN production. Out of 37, eight strains showed phosphate solubilization ranging from 13 to 24 µg/ml. eighteen strains produced indole acetic acid ranging from 5 to 19 µg/ml.
This study identified specific traits in the isolated rhizobacteria which make them good candidates as PGPR and might contribute to enhance growth of crop plants. This information is of general interest and also helpful for devising strategies to manage diseases caused by in cotton and sugarcane.
植物促生根际细菌（PGPR）可用于促进植物生长并抑制植物病原体。目的：本研究旨在分离和鉴定巴基斯坦棉花和甘蔗根际的拮抗细菌，并评估它们抑制植物病原物种的能力。在63株分离物中，研究了37种不同形态型对[具体病原物种1]、[具体病原物种2]和[具体病原物种3]的拮抗活性。其中31株菌株对[具体病原物种1]的抑制率在40%至66%之间。
使用革兰氏染色和光学显微镜对具有抗真菌活性的拮抗细菌进行不同形态和生理特征的研究。它们大多为革兰氏阴性菌，初步鉴定为[具体细菌属]种。对所选菌株进行植物生长调节和抗真菌特性筛选。
我们的研究包括1000例早发冠心病患者，分为有心肌梗死病史组（n = 461）和无心肌梗死病史组（n = 539）。通过PCR-RFLP技术测定10%样本中的多态性变异，并通过高分辨率熔解法对所有受试者的多态性进行基因分型。鉴于患者处于德黑兰且首次发生心肌梗死这两种情况，在回顾性队列阶段对1000例先前随访过的研究样本中的640例进行了长期主要不良心脏事件（MACE）评估。
四株细菌能够产生几丁质酶，另外四株细菌显示出蛋白酶产生。十株菌株HCN产生呈阳性。在37株中，八株菌株的解磷量在13至24μg/ml之间。十八株菌株产生的吲哚乙酸在5至19μg/ml之间。
本研究确定了分离的根际细菌中的特定特性，使其成为植物促生根际细菌的良好候选者，并可能有助于促进作物生长。这些信息具有普遍意义，也有助于制定防治棉花和甘蔗中由[具体病原物种]引起的疾病的策略。
