Sharma S, Sandhu D K, Bagga P S
Department of Biology, Guru Nanak Dev University, Punjab, India.
Biochem Genet. 1988 Jun;26(5-6):331-42.
Electrophoretic analysis of the distribution of various electromorphs at different beta-glucosidase zones was carried out in natural populations of A. nidulans, the A. nidulans group, and various species belonging to the genus Aspergillus from diverse geographical areas of India. The data show the existence of three segregating zones for beta-glucosidase, designated beta-GluI, beta-GluII, and beta-GluIII. All three zones are present in wild isolates of A. nidulans, and only two, i.e., beta-GluI and beta-GluIII, in the A. nidulans group and beta-GluII and beta-GluIII in different species of Aspergillus except A. terreus, A. flavus, and A. brevipes, where only beta-GluIII is present. Overall nine electromorphs are observed at beta-GluI, three at beta-GluII, and six at beta-GluIII zones, respectively. It can be concluded that there may be three structural genes for beta-glucosidase coding the three polymorphic zones in A. nidulans.
对构巢曲霉自然种群、构巢曲霉组以及来自印度不同地理区域的曲霉属各种物种,进行了不同β-葡萄糖苷酶区各种电泳变体分布的电泳分析。数据显示存在三个β-葡萄糖苷酶分离区,分别命名为β-GluI、β-GluII和β-GluIII。所有这三个区都存在于构巢曲霉的野生分离株中,而在构巢曲霉组中仅存在两个区,即β-GluI和β-GluIII,在除土曲霉、黄曲霉和短柄曲霉(仅存在β-GluIII)之外的曲霉属不同物种中存在β-GluII和β-GluIII。总体而言,在β-GluI区观察到9种电泳变体,在β-GluII区观察到3种,在β-GluIII区观察到6种。可以得出结论,可能存在三个β-葡萄糖苷酶结构基因,编码构巢曲霉中的三个多态区。