Kok T W, Varkanis G, Marmion B P, Martin J, Esterman A
Division of Medical Virology, Institute of Medical and Veterinary Science, Adelaide.
Epidemiol Infect. 1988 Dec;101(3):669-84. doi: 10.1017/s0950268800029551.
Direct and indirect antigen capture enzyme immunoassays (Ag-EIA) have been developed for the detection of Mycoplasma pneumoniae in nasopharyngeal aspirates or sputum from respiratory infection. The sensitivity of the two Ag-EIA were similar, but the indirect method using polyclonal rabbit and guinea-pig antisera was more convenient. The Ag-EIA had a detection limit of 10(4-4.5) colony-forming units/ml of sample. It was specific for M. pneumoniae and gave a low level response with M. genitalium. There were no cross-reactions with 10 other species of mycoplasmas. Tests with a wide range of bacteria and chlamydia group antigen, representing agents sometimes found in the respiratory tract, were also negative. At the current level of development, the Ag-EIA detected about 90% of specimens that were also positive for culture; 43% of specimens from culture-negative--seropositive patients gave a positive result. The overall pattern of results indicated that while antigen detection is a quick and effective substitute for the slow culture method, serological examination for specific IgM antibody is also necessary to give a complete diagnostic coverage.
已开发出直接和间接抗原捕获酶免疫测定法（Ag-EIA），用于检测呼吸道感染患者鼻咽抽吸物或痰液中的肺炎支原体。两种Ag-EIA的灵敏度相似，但使用兔和豚鼠多克隆抗血清的间接方法更方便。Ag-EIA对样品的检测限为10(4-4.5)菌落形成单位/毫升。它对肺炎支原体具有特异性，对生殖支原体的反应水平较低。与其他10种支原体没有交叉反应。用多种细菌和衣原体属群抗原进行检测，这些抗原代表呼吸道中有时发现的病原体，结果也均为阴性。在当前的发展水平下，Ag-EIA检测出约90%培养阳性的标本；43%来自培养阴性但血清学阳性患者的标本检测结果呈阳性。总体结果模式表明，虽然抗原检测是慢速培养方法的快速有效替代方法，但特异性IgM抗体的血清学检查对于全面诊断覆盖也是必要的。
